3bog: Difference between revisions
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===Snow Flea Antifreeze Protein Quasi-racemate=== | |||
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{{ABSTRACT_PUBMED_18598029}} | |||
==About this Structure== | |||
3BOG is a [[Protein complex]] structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BOG OCA]. | |||
==Reference== | |||
X-ray structure of snow flea antifreeze protein determined by racemic crystallization of synthetic protein enantiomers., Pentelute BL, Gates ZP, Tereshko V, Dashnau JL, Vanderkooi JM, Kossiakoff AA, Kent SB, J Am Chem Soc. 2008 Jul 30;130(30):9695-701. Epub 2008 Jul 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18598029 18598029] | |||
[[Category: Protein complex]] | |||
[[Category: Dashnau, J.]] | |||
[[Category: Gates, Z P.]] | |||
[[Category: Kossiakoff, A A.]] | |||
[[Category: Kurutz, J.]] | |||
[[Category: Pentelute, B L.]] | |||
[[Category: S B.H., Kent.]] | |||
[[Category: Tereshko, V.]] | |||
[[Category: Vaderkooi, J M.]] | |||
[[Category: Antifreeze protein]] | |||
[[Category: Mirror image protein]] | |||
[[Category: Quasi-racemate]] | |||
[[Category: Racemic protein crystallography]] | |||
[[Category: Sfafp]] | |||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Sep 24 10:37:39 2008'' | |||
Revision as of 07:37, 24 September 2008
Snow Flea Antifreeze Protein Quasi-racemate
Chemical protein synthesis and racemic protein crystallization were used to determine the X-ray structure of the snow flea antifreeze protein (sfAFP). Crystal formation from a racemic solution containing equal amounts of the chemically synthesized proteins d-sfAFP and l-sfAFP occurred much more readily than for l-sfAFP alone. More facile crystal formation also occurred from a quasi-racemic mixture of d-sfAFP and l-Se-sfAFP, a chemical protein analogue that contains an additional -SeCH2- moiety at one residue and thus differs slightly from the true enantiomer. Multiple wavelength anomalous dispersion (MAD) phasing from quasi-racemate crystals was then used to determine the X-ray structure of the sfAFP protein molecule. The resulting model was used to solve by molecular replacement the X-ray structure of l-sfAFP to a resolution of 0.98 A. The l-sfAFP molecule is made up of six antiparallel left-handed PPII helixes, stacked in two sets of three, to form a compact brick-like structure with one hydrophilic face and one hydrophobic face. This is a novel experimental protein structure and closely resembles a structural model proposed for sfAFP. These results illustrate the utility of total chemical synthesis combined with racemic crystallization and X-ray crystallography for determining the unknown structure of a protein.
X-ray structure of snow flea antifreeze protein determined by racemic crystallization of synthetic protein enantiomers., Pentelute BL, Gates ZP, Tereshko V, Dashnau JL, Vanderkooi JM, Kossiakoff AA, Kent SB, J Am Chem Soc. 2008 Jul 30;130(30):9695-701. Epub 2008 Jul 4. PMID:18598029
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
About this Structure
3BOG is a Protein complex structure. Full crystallographic information is available from OCA.
Reference
X-ray structure of snow flea antifreeze protein determined by racemic crystallization of synthetic protein enantiomers., Pentelute BL, Gates ZP, Tereshko V, Dashnau JL, Vanderkooi JM, Kossiakoff AA, Kent SB, J Am Chem Soc. 2008 Jul 30;130(30):9695-701. Epub 2008 Jul 4. PMID:18598029
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