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| {{STRUCTURE_2ek8| PDB=2ek8 | SCENE= }} | | {{STRUCTURE_2ek8| PDB=2ek8 | SCENE= }} |
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| '''Aminopeptidase from Aneurinibacillus sp. strain AM-1'''
| | ===Aminopeptidase from Aneurinibacillus sp. strain AM-1=== |
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| ==Overview==
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| To elucidate the structure and molecular mechanism of a characteristic proline-specific aminopeptidase produced by the thermophile Aneurinibacillus sp. strain AM-1, its gene was cloned and the recombinant protein was overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 1.8 A resolution from the recombinant aminopeptidase crystal. The crystals belong to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 93.62, b = 68.20, c = 76.84 A. A complete data set was also obtained from crystals of SeMet-substituted aminopeptidase. Data in the resolution range 20-2.1 A from the MAD data set from the SeMet-substituted crystal were used for phase determination.
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| ==About this Structure== | | ==About this Structure== |
| 2EK8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EK8 OCA]. | | 2EK8 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EK8 OCA]. |
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| ==Reference== | | ==Reference== |
| Overexpression, purification, crystallization and preliminary X-ray crystallographic studies of a proline-specific aminopeptidase from Aneurinibacillus sp. strain AM-1., Akioka M, Nakano H, Horikiri A, Tsujimoto Y, Matsui H, Shimizu T, Nakatsu T, Kato H, Watanabe K, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Dec 1;62(Pt, 12):1266-8. Epub 2006 Nov 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17142913 17142913]
| | <ref group="xtra">PMID:17142913</ref><references group="xtra"/> |
| [[Category: Bacteria]] | | [[Category: Bacteria]] |
| [[Category: Single protein]]
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| [[Category: Akioka, M.]] | | [[Category: Akioka, M.]] |
| [[Category: Nakano, H.]] | | [[Category: Nakano, H.]] |
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| [[Category: Hydrolase]] | | [[Category: Hydrolase]] |
| [[Category: Metalloproteinase]] | | [[Category: Metalloproteinase]] |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 02:40:23 2008'' | | |
| | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 11:43:26 2009'' |
