3d91: Difference between revisions

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[[Image:3d91.~~jpg~~|left|200px]]

[[Image:3d91.png|left|200px]]

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~~'''~~Human renin in complex with remikiren~~'''~~

===Human renin in complex with remikiren===

~~==Overview==~~

Human renin has been expressed in Sf9 and CHO cells using two different gene constructs. The first construct contained a foreign signal peptide fused directly to the sequence encoding mature renin, whereas the second construct harbors the sequence for preprorenin. Prorenin was produced in significantly higher amounts than the mature enzyme expressed without its propeptide in both expression systems. Both directly expressed mature renin and proteolytically derived active renin have been purified and cocrystallized with the renin inhibitor Ro 42-5892. The 3D structure has been solved for both versions and demonstrates identity despite different glycosylation and different N termini.

==Disease==

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==About this Structure==

3D91 is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D91 OCA].

3D91 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D91 OCA].

==Reference==

Recombinant human renin produced in different expression systems: biochemical properties and 3D structure., Mathews S, Dobeli H, Pruschy M, Bosser R, D'Arcy A, Oefner C, Zulauf M, Gentz R, Breu V, Matile H, Schlaeger J, Fischli W, Protein Expr Purif. 1996 Feb;7(1):81-91. PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~9172787 9172787]~~

[[Category: Homo sapiens]]

[[Category: Renin]]

~~[[Category: Single protein]]~~

[[Category: Bezencon, O.]]

[[Category: Bur, D.]]

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[[Category: Secreted]]

[[Category: Zymogen]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jun 18 12~~:13:~~09 2008~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 14:31:39 2009''

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-[[Image:3d91.jpg|left|200px]]
+{{Seed}}
+[[Image:3d91.png|left|200px]]
 <!--
@@ Line 9: / Line 10: @@
 {{STRUCTURE_3d91|  PDB=3d91  |  SCENE=  }}
-'''Human renin in complex with remikiren'''
+===Human renin in complex with remikiren===
-==Overview==
-Human renin has been expressed in Sf9 and CHO cells using two different gene constructs. The first construct contained a foreign signal peptide fused directly to the sequence encoding mature renin, whereas the second construct harbors the sequence for preprorenin. Prorenin was produced in significantly higher amounts than the mature enzyme expressed without its propeptide in both expression systems. Both directly expressed mature renin and proteolytically derived active renin have been purified and cocrystallized with the renin inhibitor Ro 42-5892. The 3D structure has been solved for both versions and demonstrates identity despite different glycosylation and different N termini.
 ==Disease==
@@ Line 19: / Line 17: @@
 ==About this Structure==
-D91 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D91 OCA].
+D91 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D91 OCA].
 ==Reference==
-Recombinant human renin produced in different expression systems: biochemical properties and 3D structure., Mathews S, Dobeli H, Pruschy M, Bosser R, D'Arcy A, Oefner C, Zulauf M, Gentz R, Breu V, Matile H, Schlaeger J, Fischli W, Protein Expr Purif. 1996 Feb;7(1):81-91. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9172787 9172787]
+<ref group="xtra">PMID:9172787</ref><references group="xtra"/>
 [[Category: Homo sapiens]]
 [[Category: Renin]]
-[[Category: Single protein]]
 [[Category: Bezencon, O.]]
 [[Category: Bur, D.]]
@@ Line 45: / Line 42: @@
 [[Category: Secreted]]
 [[Category: Zymogen]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:13:09 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 14:31:39 2009''