2bdz: Difference between revisions

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[[Image:2bdz.jpg|left|200px]]
{{Seed}}
[[Image:2bdz.png|left|200px]]


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{{STRUCTURE_2bdz|  PDB=2bdz  |  SCENE=  }}  
{{STRUCTURE_2bdz|  PDB=2bdz  |  SCENE=  }}  


'''Mexicain from Jacaratia mexicana'''
===Mexicain from Jacaratia mexicana===


==Overview==
Mexicain is a 23.7 kDa papain-like cysteine protease from the tropical plant Jacaratia mexicana. Extracted as a mix of proteases from the latex of the fruit, mexicain is isolated after cation-exchange chromatography as the most abundant product. The purified product inhibited with E-64 was crystallized by sitting-drop vapour diffusion in the presence of ethanolamine. Cryoprotected crystals diffracted X-rays from a home source to 1.98 A and belong to the monoclinic space group P2(1), with unit-cell parameters a = 57.36, b = 90.45, c = 80.39 A, beta = 92.64 degrees . The asymmetric unit contains four molecules of mexicain, with a corresponding crystal volume per protein weight (V(M)) of 2.24 A(3) Da(-1) and a solvent content of 45% by volume. A molecular-replacement model has been determined and refinement is in progress.


==About this Structure==
==About this Structure==
2BDZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Jacaratia_mexicana Jacaratia mexicana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BDZ OCA].  
2BDZ is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Jacaratia_mexicana Jacaratia mexicana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BDZ OCA].  


==Reference==
==Reference==
Purification, crystallization and preliminary X-ray analysis of mexicain., Oliver-Salvador MC, Gonzalez-Ramirez LA, Gavira JA, Soriano-Garcia M, Garcia-Ruiz JM, Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2058-60. Epub 2004, Oct 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15502326 15502326]
<ref group="xtra">PMID:15502326</ref><references group="xtra"/>
[[Category: Jacaratia mexicana]]
[[Category: Jacaratia mexicana]]
[[Category: Single protein]]
[[Category: Garcia-Ruiz, J M.]]
[[Category: Garcia-Ruiz, J M.]]
[[Category: Gavira, J A.]]
[[Category: Gavira, J A.]]
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[[Category: Papain-like]]
[[Category: Papain-like]]
[[Category: Peptidase_c1]]
[[Category: Peptidase_c1]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 20:09:38 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 18:48:22 2009''

Revision as of 16:48, 16 February 2009

File:2bdz.png

Template:STRUCTURE 2bdz

Mexicain from Jacaratia mexicana

About this Structure

2BDZ is a 4 chains structure of sequences from Jacaratia mexicana. Full crystallographic information is available from OCA.

Reference

  1. Oliver-Salvador MC, Gonzalez-Ramirez LA, Gavira JA, Soriano-Garcia M, Garcia-Ruiz JM. Purification, crystallization and preliminary X-ray analysis of mexicain. Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2058-60. Epub 2004, Oct 20. PMID:15502326 doi:10.1107/S0907444904021638

Page seeded by OCA on Mon Feb 16 18:48:22 2009

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