2dep: Difference between revisions

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[[Image:2dep.~~jpg~~|left|200px]]

[[Image:2dep.png|left|200px]]

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~~'''~~Crystal Structure of xylanase B from Clostridium stercorarium F9~~'''~~

===Crystal Structure of xylanase B from Clostridium stercorarium F9===

~~==Overview==~~

Recombinant mature xylanase B from Clostridium stercorarium has been prepared and crystallized by the sitting-drop vapour-diffusion method using 4 mg ml(-1) purified enzyme, 10.3%(w/v) polyethylene glycol 1500, 8.6%(v/v) glycerol and 0.34 M non-detergent sulfobetaine 195. A suitable crystal grew after incubation for ten weeks at 293 K. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 64.76, b = 96.60, c = 138.44 A. X-ray diffraction data were collected to 1.80 A resolution.

==About this Structure==

2DEP is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Clostridium_stercorarium Clostridium stercorarium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DEP OCA].

2DEP is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Clostridium_stercorarium Clostridium stercorarium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DEP OCA].

==Reference==

Crystallization and preliminary X-ray analysis of xylanase B from Clostridium stercorarium., Nishimoto M, Fushinobu S, Miyanaga A, Wakagi T, Shoun H, Sakka K, Ohmiya K, Nirasawa S, Kitaoka M, Hayashi K, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):~~342-3. Epub 2004, Jan 23.~~ PMID:~~[http~~://~~www.ncbi.nlm.nih.gov/pubmed/14747719 14747719]~~

[[Category: Clostridium stercorarium]]

~~[[Category: Single protein]]~~

[[Category: Fushinobu, S.]]

[[Category: Hayashi, K.]]

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[[Category: Structural genomic]]

[[Category: Xylan degradation]]

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May 4 00~~:17:~~07 2008~~''

''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 04:49:41 2009''

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-[[Image:2dep.jpg|left|200px]]
+{{Seed}}
+[[Image:2dep.png|left|200px]]
 <!--
@@ Line 9: / Line 10: @@
 {{STRUCTURE_2dep|  PDB=2dep  |  SCENE=  }}
-'''Crystal Structure of xylanase B from Clostridium stercorarium F9'''
+===Crystal Structure of xylanase B from Clostridium stercorarium F9===
-==Overview==
-Recombinant mature xylanase B from Clostridium stercorarium has been prepared and crystallized by the sitting-drop vapour-diffusion method using 4 mg ml(-1) purified enzyme, 10.3%(w/v) polyethylene glycol 1500, 8.6%(v/v) glycerol and 0.34 M non-detergent sulfobetaine 195. A suitable crystal grew after incubation for ten weeks at 293 K. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 64.76, b = 96.60, c = 138.44 A. X-ray diffraction data were collected to 1.80 A resolution.
 ==About this Structure==
-DEP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Clostridium_stercorarium Clostridium stercorarium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DEP OCA].
+DEP is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Clostridium_stercorarium Clostridium stercorarium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DEP OCA].
 ==Reference==
-Crystallization and preliminary X-ray analysis of xylanase B from Clostridium stercorarium., Nishimoto M, Fushinobu S, Miyanaga A, Wakagi T, Shoun H, Sakka K, Ohmiya K, Nirasawa S, Kitaoka M, Hayashi K, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):342-3. Epub 2004, Jan 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14747719 14747719]
+<ref group="xtra">PMID:14747719</ref><ref group="xtra">PMID:15914908</ref><ref group="xtra">PMID:16233324</ref><references group="xtra"/>
 [[Category: Clostridium stercorarium]]
-[[Category: Single protein]]
 [[Category: Fushinobu, S.]]
 [[Category: Hayashi, K.]]
@@ Line 33: / Line 30: @@
 [[Category: Structural genomic]]
 [[Category: Xylan degradation]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 00:17:07 2008''
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 04:49:41 2009''