2ab6: Difference between revisions

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[[Image:2ab6.gif|left|200px]]
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[[Image:2ab6.png|left|200px]]


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{{STRUCTURE_2ab6|  PDB=2ab6  |  SCENE=  }}  
{{STRUCTURE_2ab6|  PDB=2ab6  |  SCENE=  }}  


'''HUMAN GLUTATHIONE S-TRANSFERASE M2-2 (E.C.2.5.1.18) complexed with S-METHYLGLUTATHIONE'''
===HUMAN GLUTATHIONE S-TRANSFERASE M2-2 (E.C.2.5.1.18) complexed with S-METHYLGLUTATHIONE===


==Overview==
Human glutathione-S-transferase M2-2 (hGSTM2-2) was expressed in Escherichia coli and purified by GSH-affinity chromatography. The recombinant enzyme and the protein isolated from human tissue were indistinguishable based on physicochemical, enzymatic and immunological criteria. The catalytically active dimeric hGSTM2-2 was crystallized without GSH or other active-site ligands in two crystal forms. Diffraction from form A crystals extends to 2.5 A and is consistent with the space group P21 (a = 53.9, b = 81.5, c = 55.6 A, beta = 109.26 A) with two monomers in the asymmetric unit. Diffraction from form B crystals extends to 3 A and is consistent with a space group P212121 (a = 57.2, b = 80.7, c = 225.9 A) with two dimers in the asymmetric unit. This is the first report of ligand-free mu-class GST crystals, and a comparison with liganded complexes will provide insight into the structural consequences of substrate binding which are thought to be important for catalysis.


==About this Structure==
==About this Structure==
2AB6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB6 OCA].  
2AB6 is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2AB6 OCA].  


==Reference==
==Reference==
Expression, crystallization and preliminary X-ray analysis of ligand-free human glutathione S-transferase M2-2., Patskovska LN, Fedorov AA, Patskovsky YV, Almo SC, Listowsky I, Acta Crystallogr D Biol Crystallogr. 1998 May 1;54(Pt 3):458-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9761928 9761928]
<ref group="xtra">PMID:9761928</ref><ref group="xtra">PMID:8182750</ref><ref group="xtra">PMID:2034681</ref><references group="xtra"/>
[[Category: Glutathione transferase]]
[[Category: Glutathione transferase]]
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Almo, S C.]]
[[Category: Almo, S C.]]
[[Category: Listowsky, I.]]
[[Category: Listowsky, I.]]
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[[Category: S-methylglutathione]]
[[Category: S-methylglutathione]]
[[Category: Transferase]]
[[Category: Transferase]]
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Revision as of 04:30, 17 February 2009

File:2ab6.png

Template:STRUCTURE 2ab6

HUMAN GLUTATHIONE S-TRANSFERASE M2-2 (E.C.2.5.1.18) complexed with S-METHYLGLUTATHIONE

About this Structure

2AB6 is a 4 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

  1. Patskovska LN, Fedorov AA, Patskovsky YV, Almo SC, Listowsky I. Expression, crystallization and preliminary X-ray analysis of ligand-free human glutathione S-transferase M2-2. Acta Crystallogr D Biol Crystallogr. 1998 May 1;54(Pt 3):458-60. PMID:9761928
  2. Raghunathan S, Chandross RJ, Kretsinger RH, Allison TJ, Penington CJ, Rule GS. Crystal structure of human class mu glutathione transferase GSTM2-2. Effects of lattice packing on conformational heterogeneity. J Mol Biol. 1994 May 20;238(5):815-32. PMID:8182750 doi:https://dx.doi.org/10.1006/jmbi.1994.1336
  3. Vorachek WR, Pearson WR, Rule GS. Cloning, expression, and characterization of a class-mu glutathione transferase from human muscle, the product of the GST4 locus. Proc Natl Acad Sci U S A. 1991 May 15;88(10):4443-7. PMID:2034681

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