2gyk: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,332,843 edits
No edit summary
Line 1: Line 1:
[[Image:2gyk.jpg|left|200px]]
{{Seed}}
[[Image:2gyk.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2gyk|  PDB=2gyk  |  SCENE=  }}  
{{STRUCTURE_2gyk|  PDB=2gyk  |  SCENE=  }}  


'''Crystal structure of the complex of the Colicin E9 DNase domain with a mutant immunity protein, IMME9 (D51A)'''
===Crystal structure of the complex of the Colicin E9 DNase domain with a mutant immunity protein, IMME9 (D51A)===


==Overview==
The crystal structure of the cytotoxic endonuclease domain from the bacterial toxin colicin E9 in complex with its cognate immunity protein Im9 reveals that the inhibitor does not bind at the active site, the core of which comprises the HNH motif found in intron-encoded homing endonucleases, but rather at an adjacent position leaving the active site exposed yet unable to bind DNA because of steric and electrostatic clashes with incoming substrate. Although its mode of action is unorthodox, Im9 is a remarkably effective inhibitor since it folds within milliseconds and then associates with its target endonuclease at the rate of diffusion to form an inactive complex with sub-femtomolar binding affinity. This hyperefficient mechanism of inhibition could be well suited to other toxic enzyme systems, particularly where the substrate is a polymer extending beyond the boundaries of the active site.


==About this Structure==
==About this Structure==
2GYK is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GYK OCA].  
2GYK is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GYK OCA].  


==Reference==
==Reference==
Structural and mechanistic basis of immunity toward endonuclease colicins., Kleanthous C, Kuhlmann UC, Pommer AJ, Ferguson N, Radford SE, Moore GR, James R, Hemmings AM, Nat Struct Biol. 1999 Mar;6(3):243-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10074943 10074943]
<ref group="xtra">PMID:10074943</ref><references group="xtra"/>
[[Category: Deoxyribonuclease I]]
[[Category: Deoxyribonuclease I]]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Protein complex]]
[[Category: Hemmings, A M.]]
[[Category: Hemmings, A M.]]
[[Category: Kolade, O O.]]
[[Category: Kolade, O O.]]
Line 31: Line 28:
[[Category: Hnh-motif]]
[[Category: Hnh-motif]]
[[Category: Protein-protein complex]]
[[Category: Protein-protein complex]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:39:32 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 10:10:36 2009''

Revision as of 08:10, 17 February 2009

File:2gyk.png

Template:STRUCTURE 2gyk

Crystal structure of the complex of the Colicin E9 DNase domain with a mutant immunity protein, IMME9 (D51A)

About this Structure

2GYK is a 4 chains structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

  1. Kleanthous C, Kuhlmann UC, Pommer AJ, Ferguson N, Radford SE, Moore GR, James R, Hemmings AM. Structural and mechanistic basis of immunity toward endonuclease colicins. Nat Struct Biol. 1999 Mar;6(3):243-52. PMID:10074943 doi:https://dx.doi.org/10.1038/6683

Page seeded by OCA on Tue Feb 17 10:10:36 2009

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=2gyk&oldid=878630"