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[[Image:2gft.gif|left|200px]]
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[[Image:2gft.png|left|200px]]


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{{STRUCTURE_2gft|  PDB=2gft  |  SCENE=  }}  
{{STRUCTURE_2gft|  PDB=2gft  |  SCENE=  }}  


'''Crystal structure of the E263A nucleophile mutant of Bacillus licheniformis endo-beta-1,4-galactanase in complex with galactotriose'''
===Crystal structure of the E263A nucleophile mutant of Bacillus licheniformis endo-beta-1,4-galactanase in complex with galactotriose===


==Overview==
The beta-1,4-galactanase from Bacillus licheniformis (BLGAL) is a plant cell-wall-degrading enzyme involved in the hydrolysis of beta-1,4-galactan in the hairy regions of pectin. The crystal structure of BLGAL was determined by molecular replacement both alone and in complex with the products galactobiose and galactotriose, catching a first crystallographic glimpse of fragments of beta-1,4-galactan. As expected for an enzyme belonging to GH-53, the BLGAL structure reveals a (betaalpha)(8)-barrel architecture. However, BLGAL betaalpha-loops 2, 7 and 8 are long in contrast to the corresponding loops in structures of fungal galactanases determined previously. The structure of BLGAL additionally shows a calcium ion linking the long betaalpha-loops 7 and 8, which replaces a disulphide bridge in the fungal galactanases. Compared to the substrate-binding subsites predicted for Aspergillus aculeatus galactanase (AAGAL), two additional subsites for substrate binding are found in BLGAL, -3 and -4. A comparison of the pattern of galactan and galactooligosaccharides degradation by AAGAL and BLGAL shows that, although both are most active on substrates with a high degree of polymerization, AAGAL can degrade galactotriose and galactotetraose efficiently, whereas BLGAL prefers longer oligosaccharides and cannot hydrolyze galactotriose to any appreciable extent. This difference in substrate preference can be explained structurally by the presence of the extra subsites -3 and -4 in BLGAL.


==About this Structure==
==About this Structure==
2GFT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GFT OCA].  
2GFT is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GFT OCA].  


==Reference==
==Reference==
The structure of endo-beta-1,4-galactanase from Bacillus licheniformis in complex with two oligosaccharide products., Ryttersgaard C, Le Nours J, Lo Leggio L, Jorgensen CT, Christensen LL, Bjornvad M, Larsen S, J Mol Biol. 2004 Jul 30;341(1):107-17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15312766 15312766]
<ref group="xtra">PMID:15312766</ref><references group="xtra"/>
[[Category: Arabinogalactan endo-1,4-beta-galactosidase]]
[[Category: Arabinogalactan endo-1,4-beta-galactosidase]]
[[Category: Bacillus licheniformis]]
[[Category: Bacillus licheniformis]]
[[Category: Single protein]]
[[Category: Christensen, L L.H.]]
[[Category: Christensen, L L.H.]]
[[Category: Jorgensen, C T.]]
[[Category: Jorgensen, C T.]]
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[[Category: Nucleophile inactive mutant]]
[[Category: Nucleophile inactive mutant]]
[[Category: Protein-oligosaccharide complex]]
[[Category: Protein-oligosaccharide complex]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 05:03:33 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 13:38:12 2009''

Revision as of 11:38, 17 February 2009

File:2gft.png

Template:STRUCTURE 2gft

Crystal structure of the E263A nucleophile mutant of Bacillus licheniformis endo-beta-1,4-galactanase in complex with galactotriose

About this Structure

2GFT is a 2 chains structure of sequences from Bacillus licheniformis. Full crystallographic information is available from OCA.

Reference

  1. Ryttersgaard C, Le Nours J, Lo Leggio L, Jorgensen CT, Christensen LL, Bjornvad M, Larsen S. The structure of endo-beta-1,4-galactanase from Bacillus licheniformis in complex with two oligosaccharide products. J Mol Biol. 2004 Jul 30;341(1):107-17. PMID:15312766 doi:https://dx.doi.org/10.1016/j.jmb.2004.05.017

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