2d3q: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
Line 1: Line 1:
[[Image:2d3q.gif|left|200px]]
{{Seed}}
[[Image:2d3q.png|left|200px]]


<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2d3q|  PDB=2d3q  |  SCENE=  }}  
{{STRUCTURE_2d3q|  PDB=2d3q  |  SCENE=  }}  


'''Crystal Structure of a Decolorizing Peroxidase (DyP) That Catalyses the Biological Oxidation of Anthraquinone Derivatives'''
===Crystal Structure of a Decolorizing Peroxidase (DyP) That Catalyses the Biological Oxidation of Anthraquinone Derivatives===


==Overview==
The dye-decolorizing peroxidase DyP is a key enzyme in the decolorizing fungus Thanatephorus cucumeris Dec 1 that degrades azo and antraquinone dyes. The gene dyp from T. cucumeris Dec 1, which has low homology to other peroxidase genes, was cloned and transformed into Aspergillus oryzae and glycosylated DyP was expressed at high levels. Purified DyP was deglycosylated using GST Endo F1 and then crystallized in a strong magnetic field (10 T) at 283 K using ammonium sulfate as precipitant. X-ray diffraction data to 2.96 A resolution collected from a native crystal at the Photon Factory (Tsukuba, Japan) showed that the crystal belonged to the hexagonal space group P6(5)22, with unit-cell parameters a = b = 136.15, c = 363.46 A. The asymmetric unit of the crystal contained four DyP molecules, with a corresponding Matthews coefficient (V(M)) of 2.50 A(3) Da(-1) and a solvent content of 51%. Heavy-atom derivatives of DyP have been obtained and electron-density maps have been calculated. The haem is visible and continuous electron density between the haem and protein clearly indicates the location of the proximal histidine ligand.


==About this Structure==
==About this Structure==
2D3Q is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thanatephorus_cucumeris Thanatephorus cucumeris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D3Q OCA].  
2D3Q is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Thanatephorus_cucumeris Thanatephorus cucumeris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D3Q OCA].  


==Reference==
==Reference==
A unique dye-decolorizing peroxidase, DyP, from Thanatephorus cucumeris Dec 1: heterologous expression, crystallization and preliminary X-ray analysis., Sato T, Hara S, Matsui T, Sazaki G, Saijo S, Ganbe T, Tanaka N, Sugano Y, Shoda M, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):149-52. Epub 2003, Dec 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14684913 14684913]
<ref group="xtra">PMID:14684913</ref><references group="xtra"/>
[[Category: Single protein]]
[[Category: Thanatephorus cucumeris]]
[[Category: Thanatephorus cucumeris]]
[[Category: Sato, T.]]
[[Category: Sato, T.]]
Line 26: Line 23:
[[Category: Sugano, Y.]]
[[Category: Sugano, Y.]]
[[Category: Strands and helix]]
[[Category: Strands and helix]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May  3 23:38:56 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 17:15:19 2009''

Revision as of 15:15, 17 February 2009

File:2d3q.png

Template:STRUCTURE 2d3q

Crystal Structure of a Decolorizing Peroxidase (DyP) That Catalyses the Biological Oxidation of Anthraquinone Derivatives

About this Structure

2D3Q is a 2 chains structure of sequences from Thanatephorus cucumeris. Full crystallographic information is available from OCA.

Reference

  1. Sato T, Hara S, Matsui T, Sazaki G, Saijo S, Ganbe T, Tanaka N, Sugano Y, Shoda M. A unique dye-decolorizing peroxidase, DyP, from Thanatephorus cucumeris Dec 1: heterologous expression, crystallization and preliminary X-ray analysis. Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):149-52. Epub 2003, Dec 18. PMID:14684913

Page seeded by OCA on Tue Feb 17 17:15:19 2009

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=2d3q&oldid=897425"