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[[Image:2oqc.jpg|left|200px]]
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[[Image:2oqc.png|left|200px]]


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{{STRUCTURE_2oqc|  PDB=2oqc  |  SCENE=  }}  
{{STRUCTURE_2oqc|  PDB=2oqc  |  SCENE=  }}  


'''Crystal Structure of Penicillin V acylase from Bacillus subtilis'''
===Crystal Structure of Penicillin V acylase from Bacillus subtilis===


==Overview==
Penicillin acylase proteins are amidohydrolase enzymes that cleave penicillins at the amide bond connecting the side chain to their beta-lactam nucleus. An unannotated protein from Bacillus subtilis has been expressed in Escherichia coli, purified and confirmed to possess penicillin V acylase activity. The protein was crystallized using the hanging-drop vapour-diffusion method from a solution containing 4 M sodium formate in 100 mM Tris-HCl buffer pH 8.2. Diffraction data were collected under cryogenic conditions to a spacing of 2.5 A. The crystals belonged to the orthorhombic space group C222(1), with unit-cell parameters a = 111.0, b = 308.0, c = 56.0 A. The estimated Matthews coefficient was 3.23 A3 Da(-1), corresponding to 62% solvent content. The structure has been solved using molecular-replacement methods with B. sphaericus penicillin V acylase (PDB code 2pva) as the search model.


==About this Structure==
==About this Structure==
2OQC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OQC OCA].  
2OQC is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OQC OCA].  


==Reference==
==Reference==
Cloning, purification, crystallization and preliminary structural studies of penicillin V acylase from Bacillus subtilis., Rathinaswamy P, Pundle AV, Prabhune AA, Sivaraman H, Brannigan JA, Dodson GG, Suresh CG, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jul 1;61(Pt, 7):680-3. Epub 2005 Jun 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16511127 16511127]
<ref group="xtra">PMID:16511127</ref><references group="xtra"/>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Penicillin amidase]]
[[Category: Penicillin amidase]]
[[Category: Single protein]]
[[Category: Brannigan, J A.]]
[[Category: Brannigan, J A.]]
[[Category: Dodson, G G.]]
[[Category: Dodson, G G.]]
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[[Category: Ntn-hydrolase]]
[[Category: Ntn-hydrolase]]
[[Category: Penicillin v acylase]]
[[Category: Penicillin v acylase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 17:59:48 2009''

Revision as of 15:59, 17 February 2009

File:2oqc.png

Template:STRUCTURE 2oqc

Crystal Structure of Penicillin V acylase from Bacillus subtilis

About this Structure

2OQC is a 2 chains structure of sequences from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

  1. Rathinaswamy P, Pundle AV, Prabhune AA, Sivaraman H, Brannigan JA, Dodson GG, Suresh CG. Cloning, purification, crystallization and preliminary structural studies of penicillin V acylase from Bacillus subtilis. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jul 1;61(Pt, 7):680-3. Epub 2005 Jun 15. PMID:16511127 doi:10.1107/S1744309105017987

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