2vpr: Difference between revisions

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[[Image:2vpr.jpg|left|200px]]
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[[Image:2vpr.png|left|200px]]


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{{STRUCTURE_2vpr|  PDB=2vpr  |  SCENE=  }}  
{{STRUCTURE_2vpr|  PDB=2vpr  |  SCENE=  }}  


'''TET REPRESSOR CLASS H IN COMPLEX WITH 5A,6-ANHYDROTETRACYCLINE-MG'''
===TET REPRESSOR CLASS H IN COMPLEX WITH 5A,6-ANHYDROTETRACYCLINE-MG===


==Overview==
The tetracycline repressor (TetR) regulates the most abundant resistance mechanism against the antibiotic tetracycline in grain-negative bacteria. The TetR protein and its mutants are commonly used as control elements to regulate gene expression in higher eukaryotes. We present the crystal structure of the TetR homodimer in complex with its palindromic DNA operator at 2.5 A resolution. Comparison to the structure of TetR in complex with the inducer tetracycline-Mg2+ allows the mechanism of induction to be deduced. Inducer binding in the repressor core initiates conformational changes starting with C-terminal unwinding and shifting of the short helix a6 in each monomer. This forces a pendulum-like motion of helix a4, which increases the separation of the attached DNA binding domains by 3 A, abolishing the affinity of TetR for its operator DNA.


==About this Structure==
==About this Structure==
2VPR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VPR OCA].  
2VPR is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VPR OCA].  


==Reference==
==Reference==
Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system., Orth P, Schnappinger D, Hillen W, Saenger W, Hinrichs W, Nat Struct Biol. 2000 Mar;7(3):215-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10700280 10700280]
<ref group="xtra">PMID:10700280</ref><ref group="xtra">PMID:8153629</ref><references group="xtra"/>
[[Category: Pasteurella multocida]]
[[Category: Pasteurella multocida]]
[[Category: Single protein]]
[[Category: Hinrichs, W.]]
[[Category: Hinrichs, W.]]
[[Category: Palm, G.]]
[[Category: Palm, G.]]
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[[Category: Transcription regulation]]
[[Category: Transcription regulation]]
[[Category: Transcription regulator]]
[[Category: Transcription regulator]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:35:12 2008''
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 00:29:39 2009''

Revision as of 22:29, 17 February 2009

File:2vpr.png

Template:STRUCTURE 2vpr

TET REPRESSOR CLASS H IN COMPLEX WITH 5A,6-ANHYDROTETRACYCLINE-MG

About this Structure

2VPR is a 1 chain structure of sequence from Pasteurella multocida. Full crystallographic information is available from OCA.

Reference

  1. Orth P, Schnappinger D, Hillen W, Saenger W, Hinrichs W. Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system. Nat Struct Biol. 2000 Mar;7(3):215-9. PMID:10700280 doi:10.1038/73324
  2. Hinrichs W, Kisker C, Duvel M, Muller A, Tovar K, Hillen W, Saenger W. Structure of the Tet repressor-tetracycline complex and regulation of antibiotic resistance. Science. 1994 Apr 15;264(5157):418-20. PMID:8153629

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