Sandbox313: Difference between revisions
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Lipases catalyze the hydrolysis of ester bonds in lipids by employing a chymotrypsin-like hydrolysis mechanism. The enzyme itself is made up of both <scene name='Sandbox313/1lpmalphahelicies/2'>alpha helicies</scene> and <scene name='Sandbox313/1lpmbetasheets/1'>beta sheets</scene> sheets, but the enzyme activity hinges upon an alpha-beta hydrolase fold [http://en.wikipedia.org/wiki/Alpha/beta_hydrolase_fold]. Characteristics of this fold lie in a <scene name='Sandbox313/1lpmligand/2'>catalytic triad</scene>, all the pieces of which are located on loops.These include the reactive <scene name='Sandbox313/1lpmphosphane/1'>phosphonate</scene> center, two <scene name='Sandbox313/1lpmcalcium/1'>calcium ions</scene>, and two <scene name='Sandbox313/1lpmsugars/1'>sugars</scene> on the outside of the folded molecule. | Lipases catalyze the hydrolysis of ester bonds in lipids by employing a chymotrypsin-like hydrolysis mechanism. The enzyme itself is made up of both <scene name='Sandbox313/1lpmalphahelicies/2'>alpha helicies</scene> and <scene name='Sandbox313/1lpmbetasheets/1'>beta sheets</scene> sheets, but the enzyme activity hinges upon an alpha-beta hydrolase fold [http://en.wikipedia.org/wiki/Alpha/beta_hydrolase_fold]. Characteristics of this fold lie in a <scene name='Sandbox313/1lpmligand/2'>catalytic triad</scene>, all the pieces of which are located on loops.These include the reactive <scene name='Sandbox313/1lpmphosphane/1'>phosphonate</scene> center, two <scene name='Sandbox313/1lpmcalcium/1'>calcium ions</scene>, and two <scene name='Sandbox313/1lpmsugars/1'>sugars</scene> on the outside of the folded molecule. | ||
The molecule retains its <scene name='Sandbox313/1lpmhydrophobic/1'>hydrophobic</scene> aspects when folded, making this a well- | The molecule retains its <scene name='Sandbox313/1lpmhydrophobic/1'>hydrophobic</scene> aspects when folded, making this a well-structured enzyme. | ||
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LPM OCA]. | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LPM OCA]. | ||
