NS5B: Difference between revisions
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<applet load='2HAI_catalytic3.pdb' size='600' frame='true' align='right' caption='PDB ID 2HAI' />Placeholder, type here. | <applet load='2HAI_catalytic3.pdb' size='600' frame='true' align='right' caption='PDB ID 2HAI' />Placeholder, type here. | ||
[[Image:2HAI_domains.jpg]] | |||
== ''NS5B with active site carboxylates highlighted'' == | == ''NS5B with active site carboxylates highlighted'' == | ||
<applet load='2HAI_DNA5.pdb' size='600' frame='true' align='right' caption='PDB IDs 2HAI (protein) 1RTD (DNA)' />NS5B with B form DNA from HIV Reverse Transcriptase co-crystal model. DNA was modeled in by alignment of palms domain. A beta hairpin in the thumb domain has been moved to accommodate DNA, the hairpin is modeled into the minor groove, a possible binding site. Catalytic Mg2+ ions are modeled in green, these would be coordinated by the three aspartic acid carboxylates, (D220, D318 and D319). | <applet load='2HAI_DNA5.pdb' size='600' frame='true' align='right' caption='PDB IDs 2HAI (protein) 1RTD (DNA)' />NS5B with B form DNA from HIV Reverse Transcriptase co-crystal model. DNA was modeled in by alignment of palms domain. A beta hairpin in the thumb domain has been moved to accommodate DNA, the hairpin is modeled into the minor groove, a possible binding site. Catalytic Mg2+ ions are modeled in green, these would be coordinated by the three aspartic acid carboxylates, (D220, D318 and D319). | ||
The template strand is seen entering through a gap in the fingers domain, incoming nucleotide that extends the primer lines up with the NS5B active site and duplex DNA exits the enzyme through the large central hole. | The template strand is seen entering through a gap in the fingers domain, incoming nucleotide that extends the primer lines up with the NS5B active site and duplex DNA exits the enzyme through the large central hole. | ||
[[Image:NS5B_RNA_binders.jpg]] | |||
== ''NS5B with peptide segments known to bind RNA highlighted'' == | |||
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[[Image:PolyProtein.jpg]] | [[Image:PolyProtein.jpg]] | ||
