User:Matt Whelihan: Difference between revisions
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One of the most studied of all luciferases is that of the Japanese <scene name='User:Matt_Whelihan/Rainbow_n-c/1'>Firefly</scene> ''Luciola cruciata''. This particular luciferase enzyme is located in the light emitting organ known as the lantern in the abdomen of the beetle. Firefly larvae glow green to ward off predators and adult fireflies use this mechanism of bioluminescence to attract mates. Luciferase binds ATP/Mg+ and D-luciferin and oxidizes it to Oxyluciferin with the products of one photon of yellow-green light, pyrophosphate, AMP and CO2<ref>PMID:775940</ref>. | One of the most studied of all luciferases is that of the Japanese <scene name='User:Matt_Whelihan/Rainbow_n-c/1'>Firefly</scene> ''Luciola cruciata''. This particular luciferase enzyme is located in the light emitting organ known as the lantern in the abdomen of the beetle. Firefly larvae glow green to ward off predators and adult fireflies use this mechanism of bioluminescence to attract mates. Luciferase binds ATP/Mg+ and D-luciferin and oxidizes it to Oxyluciferin with the products of one photon of yellow-green light, pyrophosphate, AMP and CO2<ref>PMID:775940</ref>. | ||
''L. cruciata'' luciferase is a 62 kDa monooxygenase with two distinct domains. | ''L. cruciata'' luciferase is a 62 kDa monooxygenase with two distinct domains. | ||
<scene name='User:Matt_Whelihan/N-terminal_domain/1'>N-terminal domain</scene> (residues 4-436) is comprised of of an antiparalell β-barrel and two β-sheets, flanked by α-helices which forms a typical Rossmann fold [ | <scene name='User:Matt_Whelihan/N-terminal_domain/1'>N-terminal domain</scene> (residues 4-436) is comprised of of an antiparalell β-barrel and two β-sheets, flanked by α-helices which forms a typical Rossmann fold [http://images.google.com/imgres?imgurl=http://chem.csusb.edu/~dpedersn/C436/openbetarossman.jpg&imgrefurl=http://chem.csusb.edu/~dpedersn/C436/prot_struct_and_class.html&usg=__xOF4ifmAyJ5qm3UPaNKd9whKlu4=&h=539&w=719&sz=35&hl=en&start=5&um=1&tbnid=IMkWKmy0xzWcYM:&tbnh=105&tbnw=140&prev=/images%3Fq%3DRossman%2BFold%26hl%3Den%26safe%3Doff%26sa%3DN%26um%3D1] The | ||
<scene name='User:Matt_Whelihan/C-terminal_domain/2'>C-terminal domain</scene> (residues 440-544) is comprises of a separate α-β hinge<ref>PMID:8805533</ref>. | <scene name='User:Matt_Whelihan/C-terminal_domain/2'>C-terminal domain</scene> (residues 440-544) is comprises of a separate α-β hinge<ref>PMID:8805533</ref>. | ||
The <scene name='User:Matt_Whelihan/Secondary_structure/1'>structure</scene> | The <scene name='User:Matt_Whelihan/Secondary_structure/1'>structure</scene> | ||
