NS5B: Difference between revisions
No edit summary |
No edit summary |
||
| Line 5: | Line 5: | ||
<scene name='NS5B/Native_ns5b/2'>3 active site aspartates</scene> | <scene name='NS5B/Native_ns5b/2'>3 active site aspartates</scene> | ||
<scene name='NS5B/Native_ns5b/3'>Domains</scene> | <scene name='NS5B/Native_ns5b/3'>Domains</scene> | ||
<applet load='2HAI_DNA5.pdb' size='600' frame='true' align='right' caption='PDB IDs 2HAI (protein) 1RTD (DNA)' />NS5B with B form DNA from HIV Reverse Transcriptase co-crystal model. DNA was modeled in by alignment of palms domain. A beta hairpin in the thumb domain has been moved to accommodate DNA, the hairpin is modeled into the minor groove, a possible binding site. Catalytic Mg2+ ions are modeled in green, these would be coordinated by the three aspartic acid carboxylates, (D220, D318 and D319). | <applet load='2HAI_DNA5.pdb' size='600' frame='true' align='right' caption='PDB IDs 2HAI (protein) 1RTD (DNA)' />NS5B with B form DNA from HIV Reverse Transcriptase co-crystal model. DNA was modeled in by alignment of palms domain. A beta hairpin in the thumb domain has been moved to accommodate DNA, the hairpin is modeled into the minor groove, a possible binding site. Catalytic Mg2+ ions are modeled in green, these would be coordinated by the three aspartic acid carboxylates, (D220, D318 and D319). | ||
Revision as of 19:02, 20 April 2009
RNA Dependent RNA Polymerase from Hepatitis C Virus
|
Placeholder, type here.
3 active site aspartates Domains
|
NS5B with B form DNA from HIV Reverse Transcriptase co-crystal model. DNA was modeled in by alignment of palms domain. A beta hairpin in the thumb domain has been moved to accommodate DNA, the hairpin is modeled into the minor groove, a possible binding site. Catalytic Mg2+ ions are modeled in green, these would be coordinated by the three aspartic acid carboxylates, (D220, D318 and D319).
The template strand is seen entering through a gap in the fingers domain, incoming nucleotide that extends the primer lines up with the NS5B active site and duplex DNA exits the enzyme through the large central hole.
NS5B with peptide segments known to bind RNA highlighted
NS5B + LIGANDS
|
Proteopedia Page Contributors and Editors (what is this?)
Nicolas Villanueva, Kody Witham, Michal Harel, David Canner, Alexander Berchansky
