Epitopes: Difference between revisions
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Eric Martz (talk | contribs) →Epitope Prediction Servers: polishing |
Eric Martz (talk | contribs) →Epitope Prediction Servers: polishing |
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==Epitope Prediction Servers== | ==Epitope Prediction Servers== | ||
In the absence of the crystal structure of an antibody:antigen complex, a common way to identify the epitope recognized by a particular antibody is to display random peptides (for example, using phage display libraries), and then to identify the sequences of the peptides with the highest affinity for the antibody. These sequences can then be used to predict where the epitope lies on the native protein, taking into account that the epitope on the native protein may be discontinuous. In this strategy, the 3D structure of the protein antigen must be known. | In the absence of the crystal structure of an antibody:antigen complex, a common way to identify the epitope recognized by a particular antibody is to display random peptides (for example, using [http://en.wikipedia.org/wiki/Phage_display phage display libraries]), and then to identify the sequences of the peptides with the highest affinity for the antibody. These sequences can then be used to predict where the epitope lies on the native protein, taking into account that the epitope on the native protein may be discontinuous. In this strategy, the 3D structure of the protein antigen must be known. | ||
===EpiSearch=== | ===EpiSearch=== | ||
