4eef | pdb_00004eef

Crystal structure of the designed inhibitor protein F-HB80.4 in complex with the 1918 influenza virus hemagglutinin.

spinqualitylabelsall models 4eef, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4eef is a 9 chain structure with sequence from Influenza a virus and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: BMA, NAG Gene: HA, hemagglutinin (Influenza A virus) Resources: FirstGlance, OCA, RCSB, PDBsum Publication Abstract from PubMed We show that comprehensive sequence-function maps obtained by deep sequencing can be used to reprogram interaction specificity and to leapfrog over bottlenecks in affinity maturation by combining many individually small contributions not detectable in conventional approaches. We use this approach to optimize two computationally designed inhibitors against H1N1 influenza hemagglutinin and, in both cases, obtain variants with subnanomolar binding affinity. The most potent of these, a 51-residue protein, is broadly cross-reactive against all influenza group 1 hemagglutinins, including human H2, and neutralizes H1N1 viruses with a potency that rivals that of several human monoclonal antibodies, demonstrating that computational design followed by comprehensive energy landscape mapping can generate proteins with potential therapeutic utility. Optimization of affinity, specificity and function of designed influenza inhibitors using deep sequencing.,Whitehead TA, Chevalier A, Song Y, Dreyfus C, Fleishman SJ, De Mattos C, Myers CA, Kamisetty H, Blair P, Wilson IA, Baker D Nat Biotechnol. 2012 May 27;30(6):543-8. doi: 10.1038/nbt.2214. PMID:22634563 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Hemagglutinin

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