6ck0 | pdb_00006ck0

Crystal Structure of Biotin Acetyl Coenzyme A Carboxylase Synthetase from Helicobacter pylori with bound Biotinylated ATP

spinqualitylabelsall models 6ck0, resolution 2.25Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6ck0 is a 2 chain structure with sequence from Helicobacter pylori G27. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.25Å Ligands: EDO, F5D, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B5Z8D8_HELPG Publication Abstract from PubMed Helicobacter pylori, a type 1 carcinogen that causes human gastric ulcers and cancer, is a priority target of the Seattle Structural Genomics Center for Infectious Disease (SSGCID). These efforts include determining the structures of potential H. pylori therapeutic targets. Here, the purification, crystallization and X-ray structure of one such target, H. pylori biotin protein ligase (HpBPL), are reported. HpBPL catalyzes the activation of various biotin-dependent metabolic pathways, including fatty-acid synthesis, gluconeogenesis and amino-acid catabolism, and may facilitate the survival of H. pylori in the high-pH gastric mucosa. HpBPL is a prototypical bacterial biotin protein ligase, despite having less than 35% sequence identity to any reported structure in the Protein Data Bank. A biotinyl-5-ATP molecule sits in a well conserved cavity. HpBPL shares extensive tertiary-structural similarity with Mycobacterium tuberculosis biotin protein ligase (MtBPL), despite having less than 22% sequence identity. The active site of HpBPL is very similar to that of MtBPL and has the necessary residues to bind inhibitors developed for MtBPL. Co-crystal structure of Helicobacter pylori biotin protein ligase with biotinyl-5-ATP.,Ayanlade JP, Davis DE, Subramanian S, Dranow DM, Lorimer DD, Hammerson B, Myler PJ, Asojo OA Acta Crystallogr F Struct Biol Commun. 2025 Jan 1;81(Pt 1):11-18. doi: , 10.1107/S2053230X24012056. Epub 2025 Jan 1. PMID:39704719 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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