7sfa

Branchiostoma floridae fluorescent protein LanFP10A2

spinqualitylabelsall models 7sfa, resolution 1.65Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7sfa is a 4 chain structure with sequence from Branchiostoma floridae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.65Å Ligands: JBY Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function C3YRA2_BRAFL Publication Abstract from PubMed We describe an engineered violet fluorescent protein from the lancelet Branchiostoma floridae (bfVFP). This is the first example of a GFP-like fluorescent protein with a stable fluorescent chromophore lacking an imidazolinone ring; instead, it consists of oxidized tyrosine 68 flanked by glycine 67 and alanine 69. bfVFP contains the simplest chromophore reported in fluorescent proteins and was generated from the yellow protein lanFP10A2 by two synergetic mutations, S148H and C166I. The chromophore structure was confirmed crystallographically and by high-resolution mass spectrometry. The photophysical characteristics of bfVFP (323/430 nm, quantum yield 0.33, and Ec 14,300 M(-1) cm(-1) ) make it potentially useful for multicolor experiments to expand the excitation range of available FP biomarkers and Forster resonance energy transfer with blue and cyan fluorescent protein acceptors. A novel violet fluorescent protein contains a unique oxidized tyrosine as the simplest chromophore ever reported in fluorescent proteins.,Roldan-Salgado A, Muslinkina L, Pletnev S, Pletneva N, Pletnev V, Gaytan P Protein Sci. 2022 Mar;31(3):688-700. doi: 10.1002/pro.4265. Epub 2022 Jan 3. PMID:34936154 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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