9nvq | pdb_00009nvq

Structure of Nanchung-Inactive-Calmodulin in complex with Afidopyropen and calcium

spinqualitylabelsall models 9nvq, resolution 3.01Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9nvq is a 6 chain structure with sequence from Halyomorpha halys and Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.01Å Ligands: 6OU, A1B32, CA, D39, LBN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A9P0HI19_NEZVI A0A9P0MRC5_NEZVI Publication Abstract from PubMed Insecticides are vital to combating world food shortages and transmission of vector-borne human diseases. Increasing insecticide resistance necessitates discovery of novel compounds against underutilized targets. Nanchung (Nan) and Inactive (Iav), the transient receptor potential vanilloid-type (TRPV) channels in insects, likely form a heteromeric channel (Nan-Iav) and are localized in mechanosensory chordotonal organs which confer gravitaxis, hearing and proprioception. Several insecticides, such as afidopyropen (AP), target Nan-Iav through unknown mechanisms. Effective against piercing-sucking (hemipteran) insects, AP disrupts chordotonal functions preventing feeding. AP can bind to Nan alone, but only Nan-Iav exhibits channel activity with agonists including endogenous nicotinamide (NAM). Despite its importance as an insecticide target, much is unknown about Nan-Iav, such as channel assembly, modulator binding sites, and Ca(2+)-dependent regulation, hampering further insecticide development. Here we present the cryo-electron microscopy structures of hemipteran Nan-Iav with calmodulin bound in the apo state and with AP and NAM bound to cytosolic ankyrin repeat domain (ARD) interfaces. Unexpectedly, we found that Nan alone can form a pentamer, stabilized through AP-mediated ARD interactions. Our study provides molecular insights into insecticide and agonist interactions with Nan-Iav, highlighting the importance of the ARD on channel function and assembly, while also probing regulation by Ca(2+). Visualizing insecticide control of insect TRP channel function and assembly.,Fedor JG, Kandasamy R, Park CG, Suo Y, Weisel M, Rankl NB, Nesterov A, Lee SY Nat Commun. 2025 Dec 14;17(1):595. doi: 10.1038/s41467-025-67287-2. PMID:41392056 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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