3b31 | pdb_00003b31
Crystal structure of domain III of the Cricket Paralysis Virus IRES RNA
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Overview
Internal ribosome entry site (IRES) RNAs initiate protein synthesis in, eukaryotic cells by a noncanonical cap-independent mechanism. IRESes are, critical for many pathogenic viruses, but efforts to understand their, function are complicated by the diversity of IRES sequences as well as by, limited high-resolution structural information. The intergenic region, (IGR) IRESes of the Dicistroviridae viruses are powerful model systems to, begin to understand IRES function. Here we present the crystal structure, of a Dicistroviridae IGR IRES domain that interacts with the ribosome's, decoding groove. We find that this RNA domain precisely mimics the, transfer RNA anticodon-messenger RNA codon interaction, and its modeled, orientation on the ribosome helps explain translocation without peptide, bond formation. When combined with a previous structure, this work, completes the first high-resolution description of an IRES RNA and, provides insight into how RNAs can manipulate complex biological machines.
About this Structure
3B31 is a Protein complex structure of sequences from [1] with PO4 and IRI as ligands. Full crystallographic information is available from OCA.
Reference
tRNA-mRNA mimicry drives translation initiation from a viral IRES., Costantino DA, Pfingsten JS, Rambo RP, Kieft JS, Nat Struct Mol Biol. 2008 Jan;15(1):57-64. Epub 2007 Dec 23. PMID:18157151
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