Structural highlights
4ehu is a 2 chain structure with sequence from Clostridioides difficile. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Method: | X-ray diffraction, Resolution 1.6Å |
| Ligands: | ACT, ANP, MG, NA, SF4 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
HADI_CLODI Involved in the reductive branch of L-leucine fermentation. Required for the activation of (R)-2-hydroxyisocaproyl-CoA dehydratase. The reduced activator transfers one electron to the dehydratase concomitant with hydrolysis of ATP. This protein is extremely sensitive towards oxygen.[1] [2]
References
- ↑ Kim J, Darley D, Buckel W. 2-Hydroxyisocaproyl-CoA dehydratase and its activator from Clostridium difficile. FEBS J. 2005 Jan;272(2):550-61. doi: 10.1111/j.1742-4658.2004.04498.x. PMID:15654892 doi:https://dx.doi.org/10.1111/j.1742-4658.2004.04498.x
- ↑ Knauer SH, Buckel W, Dobbek H. On the ATP-dependent Activation of the Radical Enzyme (R)-2-Hydroxyisocaproyl-CoA Dehydratase. Biochemistry. 2012 Jul 24. PMID:22827463 doi:10.1021/bi300571z
