4d40

High-Resolution Structure of a Type IV Pilin from Shewanella oneidensis

spinqualitylabelsall models 4d40, resolution 1.67Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 4d40 is a 2 chain structure with sequence from Shewanella oneidensis MR-1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.669Å Ligands: NA, SO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q8EII5_SHEON Publication Abstract from PubMed BACKGROUND: Type IV pili are widely expressed among Gram-negative bacteria, where they are involved in biofilm formation, serve in the transfer of DNA, motility and in the bacterial attachment to various surfaces. Type IV pili in Shewanella oneidensis are also supposed to play an important role in extracellular electron transfer by the attachment to sediments containing electron acceptors and potentially forming conductive nanowires. RESULTS: The potential nanowire type IV pilin PilBac1 from S. oneidensis was characterized by a combination of complementary structural methods and the atomic structure was determined at a resolution of 1.67 A by X-ray crystallography. PilBac1 consists of one long N-terminal alpha-helix packed against four antiparallel beta-strands, thus revealing the core fold of type IV pilins. In the crystal, PilBac1 forms a parallel dimer with a sodium ion bound to one of the monomers. Interestingly, our PilBac1 crystal structure reveals two unusual features compared to other type IVa pilins: an unusual position of the disulfide bridge and a straight alpha-helical section, which usually exhibits a pronounced kink. This straight helix leads to a distinct packing in a filament model of PilBac1 based on an EM model of a Neisseria pilus. CONCLUSIONS: In this study we have described the first structure of a pilin from Shewanella oneidensis. The structure possesses features of the common type IV pilin core, but also exhibits significant variations in the alpha-helical part and the D-region. High-resolution structure of a type IV pilin from the metal-reducing bacterium Shewanella oneidensis.,Gorgel M, Ulstrup JJ, Boggild A, Jones NC, Hoffmann SV, Nissen P, Boesen T BMC Struct Biol. 2015 Feb 27;15(1):4. doi: 10.1186/s12900-015-0031-7. PMID:25886849 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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