5hoe

Crystal structrue of Est24, a carbohydrate acetylesterase from Sinorhizobium meliloti

spinqualitylabelsall models 5hoe, resolution 1.45Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 5hoe is a 4 chain structure with sequence from Sinorhizobium meliloti 1021. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.45Å Ligands: P6G, PO4 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q92XZ6_RHIME Publication Abstract from PubMed Carbohydrate acetylesterases, which have a highly specific role among plant-interacting bacterial species, remove the acetyl groups from plant carbohydrates. Here, we determined the crystal structure of Est24, an octameric carbohydrate acetylesterase from Sinorhizobium meliloti, at 1.45 A resolution and investigated its biochemical properties. The structure of Est24 consisted of five parallel beta strands flanked by alpha helices, which formed an octameric assembly with two distinct interfaces. The deacetylation activity of Est24 and its mutants around the substrate-binding pocket was investigated using several substrates, including glucose pentaacetate and acetyl alginate. Elucidation of the structure-function relationships of Est24 could provide valuable opportunities for biotechnological explorations. Structural and Biochemical Characterization of an Octameric Carbohydrate Acetylesterase from Sinorhizobium meliloti.,Oh C, Ryu BH, An DR, Nguyen DD, Yoo W, Kim T, Ngo TD, Kim HS, Kim KK, Kim TD FEBS Lett. 2016 Apr;590(8):1242-52. doi: 10.1002/1873-3468.12135. Epub 2016 Mar, 31. PMID:26991446 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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