5vu2

Electron cryo-microscopy of "immature" Chikungunya VLP

Loading... Status: waiting for Mol* initialization Structural highlights 5vu2 is a 24 chain structure with sequence from Chikungunya virus strain Senegal 37997. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 6.8Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A0H4CWF2_CHIKV Publication Abstract from PubMed Cleavage of the alphavirus precursor glycoprotein p62 into the E2 and E3 glycoproteins before assembly with the nucleocapsid is the key to producing fusion-competent mature spikes on alphaviruses. Here we present a cryo-EM, 6.8-A resolution structure of an "immature" Chikungunya virus in which the cleavage site has been mutated to inhibit proteolysis. The spikes in the immature virus have a larger radius and are less compact than in the mature virus. Furthermore, domains B on the E2 glycoproteins have less freedom of movement in the immature virus, keeping the fusion loops protected under domain B. In addition, the nucleocapsid of the immature virus is more compact than in the mature virus, protecting a conserved ribosome-binding site in the capsid protein from exposure. These differences suggest that the posttranslational processing of the spikes and nucleocapsid is necessary to produce infectious virus. Structural studies of Chikungunya virus maturation.,Yap ML, Klose T, Urakami A, Hasan SS, Akahata W, Rossmann MG Proc Natl Acad Sci U S A. 2017 Dec 4. pii: 1713166114. doi:, 10.1073/pnas.1713166114. PMID:29203665 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Virus coat proteins 3D structures

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