6ge1 | pdb_00006ge1

Solution structure of the r(UGGUGGU)4 RNA quadruplex

spinqualitylabelsall models 6ge1 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6ge1 is a 4 chain structure with sequence from Synthetic construct. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Uridine tetrads (U-tetrads) are a structural element encountered in RNA G-quadruplexes, for example, in the structures formed by the biologically relevant human telomeric repeat RNA. For these molecules, an unexpectedly strong stabilizing influence of a U-tetrad forming at the 3' terminus of a quadruplex was reported. Here we present the high resolution solution NMR structure of the r(UGGUGGU)4 quadruplex which, in our opinion, provides an explanation for this stabilization. Our structure features a distinctive, abrupt chain reversal just prior to the 3' uridine tetrad. Similar 'reversed U-tetrads' were already observed in the crystalline phase. However, our NMR structure coupled with extensive explicit solvent molecular dynamics (MD) simulations identifies some key features of this motif, that up to now remained overlooked. These include the presence of an exceptionally stable 2'OH to phosphate hydrogen bond, as well as, the formation of an additional K(+) binding pocket in the quadruplex groove. Unraveling the structural basis for the exceptional stability of RNA G-quadruplexes capped by a uridine tetrad at the 3' terminus.,Andralojc W, Malgowska M, Sarzynska J, Pasternak K, Szpotkowski K, Kierzek R, Gdaniec Z RNA. 2018 Oct 19. pii: rna.068163.118. doi: 10.1261/rna.068163.118. PMID:30341177 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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