6r9z | pdb_00006r9z

3D NMR solution structure of ligand peptide (Ac)EVNPPVP of Pro-Pro endopeptidase-1

spinqualitylabelsall models 6r9z Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6r9z is a 1 chain structure with sequence from Clostridioides difficile. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Solution NMR, 20 models Ligands: ACE, NH2 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Pro-Pro endopeptidase-1 (PPEP-1) is a secreted metalloprotease from the bacterial pathogen Clostridium difficile that cleaves two endogenous adhesion proteins. PPEP-1 is therefore important for bacterial motility and hence for efficient gut colonization during infection. PPEP-1 exhibits a unique specificity for Pro-Pro peptide bonds within the consensus sequence VNP downward arrowPVP. In this study, we combined information from crystal and NMR structures with mutagenesis and enzyme kinetics to investigate the mechanism and substrate specificity of PPEP-1. Our analyses revealed that the substrate-binding cleft of PPEP-1 is shaped complementary to the major conformation of the substrate in solution. We found that it possesses features that accept a tertiary amide and help discriminate P1' residues by their amide hydrogen bond-donating potential. We also noted that residues Lys-101, Trp-103, and Glu-184 are crucial for proteolytic activity. Upon substrate binding, these residues position a flexible loop over the substrate-binding cleft and modulate the second coordination sphere of the catalytic zinc ion. On the basis of these findings, we propose an induced-fit model in which pre-structured substrates are recognized, followed by substrate positioning within the active-site cleft and a concomitant increase in the Lewis acidity of the catalytic Zn2+ ion. In conclusion, our findings provide detailed structural and mechanistic insights into the substrate recognition and specificity of PPEP-1 from the common gut pathogen Clostridium difficile. Molecular determinants of the mechanism and substrate specificity of Clostridium difficile proline-proline endopeptidase-1.,Pichlo C, Juetten L, Wojtalla F, Schacherl M, Diaz D, Baumann U J Biol Chem. 2019 Jun 10. pii: RA119.009029. doi: 10.1074/jbc.RA119.009029. PMID:31182482 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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