6spp | pdb_00006spp

Structure of the Escherichia coli methionyl-tRNA synthetase variant VI298

spinqualitylabelsall models 6spp, resolution 1.49Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 6spp is a 1 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.49Å Ligands: CIT, GOL, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function SYM_ECOLI Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.[HAMAP-Rule:MF_00098] Publication Abstract from PubMed Polypeptides containing beta-amino acids are attractive tools for the design of novel proteins having unique properties of medical or industrial interest. Incorporation of beta-amino acids in vivo requires the development of efficient aminoacyl-tRNA synthetases specific of these non-canonical amino acids. Here, we have performed a detailed structural and biochemical study of the recognition and use of beta(3)-Met by Escherichia coli methionyl-tRNA synthetase (MetRS). We show that MetRS binds beta(3)-Met with a 24-fold lower affinity but catalyzes the esterification of the non-canonical amino acid onto tRNA with a rate lowered by three orders of magnitude. Accurate measurements of the catalytic parameters required careful consideration of the presence of contaminating alpha-Met in beta(3)-Met commercial samples. The 1.45 A crystal structure of the MetRS: beta(3)-Met complex shows that beta(3)-Met binds the enzyme essentially like alpha-Met, but the carboxylate moiety is mobile and not adequately positioned to react with ATP for aminoacyl adenylate formation. This study provides structural and biochemical bases for engineering MetRS with improved beta(3)-Met aminoacylation capabilities. Use of beta(3)-methionine as an amino acid substrate of Escherichia coli methionyl-tRNA synthetase.,Nigro G, Bourcier S, Lazennec-Schurdevin C, Schmitt E, Marliere P, Mechulam Y J Struct Biol. 2019 Dec 17:107435. doi: 10.1016/j.jsb.2019.107435. PMID:31862305 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Aminoacyl tRNA synthetase 3D structures

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