7bbl

Structure of human Gemin6/Gemin7/Gemin8 trimeric complex

spinqualitylabelsall models 7bbl, resolution 1.52Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7bbl is a 6 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.521Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function GEMI6_HUMAN The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. Publication Abstract from PubMed The macromolecular SMN complex facilitates the formation of Sm-class ribonucleoproteins involved in mRNA processing (UsnRNPs). While biochemical studies have revealed key activities of the SMN complex, its structural investigation is lagging behind. Here we report on the identification and structural determination of the SMN complex from the lower eukaryote Schizosaccharomyces pombe, consisting of SMN, Gemin2, 6, 7, 8 and Sm proteins. The core of the SMN complex is formed by several copies of SMN tethered through its C-terminal alpha-helices arranged with alternating polarity. This creates a central platform onto which Gemin8 binds and recruits Gemins 6 and 7. The N-terminal parts of the SMN molecules extrude via flexible linkers from the core and enable binding of Gemin2 and Sm proteins. Our data identify the SMN complex as a multivalent hub where Sm proteins are collected in its periphery to allow their joining with UsnRNA. Identification and structural analysis of the Schizosaccharomyces pombe SMN complex.,Veepaschit J, Viswanathan A, Bordonne R, Grimm C, Fischer U Nucleic Acids Res. 2021 Jul 21;49(13):7207-7223. doi: 10.1093/nar/gkab158. PMID:33754639 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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