7esi

Crystal structure of the collagenase unit of a Vibrio collagenase from Vibrio harveyi VHJR7 at 1. 8 angstrom resolution.

spinqualitylabelsall models 7esi, resolution 1.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7esi is a 3 chain structure with sequence from Vibrio harveyi VHJR7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.8Å Ligands: CA, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function A0A8B3DGT3_VIBHA Publication Abstract from PubMed The collagenases of Vibrio species, many of which are pathogens, have been regarded as an important virulence factor. However, there is little information on the structure and collagenolytic mechanism of Vibrio collagenase. Here, we report the crystal structure of the collagenase module (CM) of Vibrio collagenase VhaC and the conformation of VhaC in solution. Structural and biochemical analyses and molecular dynamics studies reveal that triple-helical collagen is initially recognized by the activator domain, followed by subsequent cleavage by the peptidase domain along with the closing movement of CM. This is different from the peptidolytic mode or the proposed collagenolysis of Clostridium collagenase. We propose a model for the integrated collagenolytic mechanism of VhaC, integrating the functions of VhaC accessory domains and its collagen degradation pattern. This study provides insight into the mechanism of bacterial collagenolysis and helps in structure-based drug design targeting of the Vibrio collagenase. Structure of Vibrio collagenase VhaC provides insight into the mechanism of bacterial collagenolysis.,Wang Y, Wang P, Cao HY, Ding HT, Su HN, Liu SC, Liu G, Zhang X, Li CY, Peng M, Li F, Li S, Chen Y, Chen XL, Zhang YZ Nat Commun. 2022 Jan 28;13(1):566. doi: 10.1038/s41467-022-28264-1. PMID:35091565 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Collagenase 3D structures

Proteopedia Page Contributors and Editors (what is this?)

OCA