7kpu | pdb_00007kpu

Crystal structure of human NatD (NAA40) bound to a bisubstrate analogue with a C-3 linker

spinqualitylabelsall models 7kpu, resolution 1.43Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 7kpu is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.43Å Ligands: ACE, BTB, GOL, NH2, SO4, WZG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function NAA40_HUMAN Responsible for the acetylation of the N-terminal residues of histones H4 and H2A. Publication Abstract from PubMed Protein N-terminal acetyltransferase D (NatD, NAA40) that specifically acetylates the alpha-N-terminus of histone H4 and H2A has been implicated in various diseases, but no inhibitor has been reported for this important enzyme. Based on the acetyl transfer mechanism of NatD, we designed and prepared a series of highly potent NatD bisubstrate inhibitors by covalently linking coenzyme A to different peptide substrates via an acetyl or propionyl spacer. The most potent bisubstrate inhibitor displayed an apparent K(i) value of 1.0 nM. Biochemical studies indicated that bisubstrate inhibitors are competitive to the peptide substrate and noncompetitive to the cofactor, suggesting that NatD undergoes an ordered Bi-Bi mechanism. We also demonstrated that these inhibitors are highly specific toward NatD, displaying about 1000-fold selectivity over other closely related acetyltransferases. High-resolution crystal structures of NatD bound to two of these inhibitors revealed the molecular basis for their selectivity and inhibition mechanism, providing a rational path for future inhibitor development. Novel Bisubstrate Inhibitors for Protein N-Terminal Acetyltransferase D.,Deng Y, Deng S, Ho YH, Gardner SM, Huang Z, Marmorstein R, Huang R J Med Chem. 2021 Jun 24;64(12):8263-8271. doi: 10.1021/acs.jmedchem.1c00141. Epub , 2021 Jun 10. PMID:34110812 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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