7pc3
The second PDZ domain of DLG1 complexed with the PDZ-binding motif of HTLV1-TAX1
Structural highlights
FunctionANXA2_HUMAN Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response.DLG1_HUMAN Essential multidomain scaffolding protein required for normal development (By similarity). Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel.[1] [2] [3] [4] [5] [6] Publication Abstract from PubMedThe human PDZome represents one of the largest globular domain families in the human proteome, with 266 instances. These globular domains typically interact with C-terminal peptide motifs found in thousands of human proteins. Despite previous efforts, not all PDZ domains have experimentally solved structures and most of their complexes remain to be solved. Here, a simple and cost-effective strategy is proposed for the crystallization of PDZ domains and their complexes. A human annexin A2 fusion tag was used as a crystallization chaperone and the structures of nine PDZ domains were solved, including five domains that had not yet been solved. Finally, these novel experimental structures were compared with AlphaFold predictions and it is speculated how predictions and experimental methods could cooperate in order to investigate the structural landscapes of entire domain families and interactomes. A scalable strategy to solve structures of PDZ domains and their complexes.,Cousido-Siah A, Carneiro L, Kostmann C, Ecsedi P, Nyitray L, Trave G, Gogl G Acta Crystallogr D Struct Biol. 2022 Apr 1;78(Pt 4):509-516. doi:, 10.1107/S2059798322001784. Epub 2022 Mar 11. PMID:35362473[7] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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