8cdt

Crystal structure of the xNup93-Nb2t VHH antibody

spinqualitylabelsall models 8cdt, resolution 1.41Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8cdt is a 1 chain structure with sequence from Vicugna pacos. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 1.41Å Ligands: GOL Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Nuclear pore complex (NPC) biogenesis is a still enigmatic example of protein self-assembly. We now introduce several cross-reacting anti-Nup nanobodies for imaging intact nuclear pore complexes from frog to human. We also report a simplified assay that directly tracks postmitotic NPC assembly with added fluorophore-labeled anti-Nup nanobodies. During interphase, NPCs are inserted into a pre-existing nuclear envelope. Monitoring this process is challenging because newly assembled NPCs are indistinguishable from pre-existing ones. We overcame this problem by inserting Xenopus-derived NPCs into human nuclear envelopes and using frog-specific anti-Nup nanobodies for detection. We further asked whether anti-Nup nanobodies could serve as NPC assembly inhibitors. Using a selection strategy against conserved epitopes, we obtained anti-Nup93, Nup98, and Nup155 nanobodies that block Nup-Nup interfaces and arrest NPC assembly. We solved structures of nanobody-target complexes and identified roles for the Nup93 alpha-solenoid domain in recruiting Nup358 and the Nup214.88.62 complex, as well as for Nup155 and the Nup98 autoproteolytic domain in NPC scaffold assembly. The latter suggests a checkpoint linking pore formation to the assembly of the Nup98-dominated permeability barrier. A checkpoint function for Nup98 in nuclear pore formation suggested by novel inhibitory nanobodies.,Sola Colom M, Fu Z, Gunkel P, Guttler T, Trakhanov S, Srinivasan V, Gregor K, Pleiner T, Gorlich D EMBO J. 2024 Jun;43(11):2198-2232. doi: 10.1038/s44318-024-00081-w. Epub 2024 Apr , 22. PMID:38649536 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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