8dts

X-ray crystal structure of AFSSFN from chaperone DNAJB8.

spinqualitylabelsall models 8dts, resolution 0.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8dts is a 12 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 0.75Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function DNJB8_HUMAN Efficient suppressor of aggregation and toxicity of disease-associated polyglutamine proteins. Publication Abstract from PubMed J-domain protein (JDP) molecular chaperones have emerged as central players that maintain a healthy proteome. The diverse members of the JDP family function as monomers/dimers and a small subset assemble into micron-sized oligomers. The oligomeric JDP members have eluded structural characterization due to their low-complexity, intrinsically disordered middle domains. This in turn, obscures the biological significance of these larger oligomers in protein folding processes. Here, we identified a short, aromatic motif within DNAJB8 that drives self-assembly through pi-pi stacking and determined its X-ray structure. We show that mutations in the motif disrupt DNAJB8 oligomerization in vitro and in cells. DNAJB8 variants that are unable to assemble bind to misfolded tau seeds more specifically and retain capacity to reduce protein aggregation in vitro and in cells. We propose a new model for DNAJB8 function in which the sequences in the low-complexity domains play distinct roles in assembly and substrate activity. DNAJB8 oligomerization is mediated by an aromatic-rich motif that is dispensable for substrate activity.,Ryder BD, Ustyantseva E, Boyer DR, Mendoza-Oliva A, Kuska MI, Wydorski PM, Macierzynska P, Morgan N, Sawaya MR, Diamond MI, Kampinga HH, Joachimiak LA Structure. 2024 Jun 6;32(6):662-678.e8. doi: 10.1016/j.str.2024.02.015. Epub 2024 , Mar 19. PMID:38508190 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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