8ojj | pdb_00008ojj

Cryo-EM structure of the DnaD-NTD tetramer

spinqualitylabelsall models 8ojj, resolution 5.47Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8ojj is a 4 chain structure with sequence from Bacillus subtilis subsp. subtilis str. 168. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 5.47Å Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function DNAD_BACSU Probable component of primosome involved in the initiation of DNA replication. Publication Abstract from PubMed Genome replication is a fundamental biological activity shared by all organisms. Chromosomal replication proceeds bidirectionally from origins, requiring the loading of two helicases, one for each replisome. However, the molecular mechanisms underpinning helicase loading at bacterial chromosome origins (oriC) are unclear. Here we investigated the essential DNA replication initiation protein DnaD in the model organism Bacillus subtilis. A set of DnaD residues required for ssDNA binding was identified, and photo-crosslinking revealed that this ssDNA binding region interacts preferentially with one strand of oriC. Biochemical and genetic data support the model that DnaD recognizes a new single-stranded DNA (ssDNA) motif located in oriC, the DnaD Recognition Element (DRE). Considered with single particle cryo-electron microscopy (cryo-EM) imaging of DnaD, we propose that the location of the DRE within oriC orchestrates strand-specific recruitment of helicase during DNA replication initiation. These findings significantly advance our mechanistic understanding of bidirectional replication from a bacterial chromosome origin. The DNA replication initiation protein DnaD recognises a specific strand of the Bacillus subtilis chromosome origin.,Winterhalter C, Pelliciari S, Stevens D, Fenyk S, Marchand E, Cronin NB, Soultanas P, Costa TRD, Ilangovan A, Murray H Nucleic Acids Res. 2023 May 22;51(9):4322-4340. doi: 10.1093/nar/gkad277. PMID:37093985 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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