8rs0 | pdb_00008rs0

Structure of RyR1 in detergent in primed state in complex with nanobody and FKBP

spinqualitylabelsall models 8rs0, resolution 3.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 8rs0 is a 12 chain structure with sequence from Oryctolagus cuniculus and Vicugna pacos. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 3.3Å Ligands: ATP, CA, CFF, ZN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function FKB1B_RABIT Has the potential to contribute to the immunosuppressive and toxic effects of FK506 and rapamycin. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides (By similarity). Publication Abstract from PubMed Ryanodine receptors (RyRs) are large Ca(2+) release channels residing in the endoplasmic or sarcoplasmic reticulum membrane. Three isoforms of RyRs have been identified in mammals, the disfunction of which has been associated with a series of life-threatening diseases. The need for large amounts of native tissue or eukaryotic cell cultures limits advances in structural studies of RyRs. Here, we report a method that utilizes nanobodies to purify RyRs from only 5 mg of total protein. The purification process, from isolated membranes to cryo-EM grade protein, is achieved within 4 h on the bench, yielding protein usable for cryo-EM analysis. This is demonstrated by solving the structures of rabbit RyR1, solubilized in detergent, reconstituted into lipid nanodiscs or liposomes, and bovine RyR2 reconstituted in nanodisc, and mouse RyR2 in detergent. The reported method facilitates structural studies of RyRs directed toward drug development and is useful in cases where the amount of starting material is limited. Rapid small-scale nanobody-assisted purification of ryanodine receptors for cryo-EM.,Li C, Willegems K, Uchanski T, Pardon E, Steyaert J, Efremov RG J Biol Chem. 2024 Sep 2;300(10):107734. doi: 10.1016/j.jbc.2024.107734. PMID:39233227 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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