9fty

Serial microseconds crystallography at ID29 using fixed-target (small foils): Proteinase K with 20 um spacing between X-ray pulses

spinqualitylabelsall models 9fty, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9fty is a 1 chain structure with sequence from Parengyodontium album. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2Å Ligands: CA, NO3 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function PRTK_PARAQ Hydrolyzes keratin at aromatic and hydrophobic residues. Publication Abstract from PubMed Serial macromolecular crystallography has become a powerful method to reveal room temperature structures of biological macromolecules and perform time-resolved studies. ID29, a flagship beamline of the ESRF 4th generation synchrotron, is the first synchrotron beamline in the world capable of delivering high brilliance microsecond X-ray pulses at high repetition rate for the structure determination of biological macromolecules at room temperature. The cardinal combination of microsecond exposure times, innovative beam characteristics and adaptable sample environment provides high quality complete data, even from an exceptionally small amount of crystalline material, enabling what we collectively term serial microsecond crystallography (SmicroX). After validating the use of different sample delivery methods with various model systems, we applied SmicroX to an integral membrane receptor, where only a few thousands diffraction images were sufficient to obtain a fully interpretable electron density map for the antagonist istradefylline-bound A(2A) receptor conformation, providing access to the antagonist binding mode. SmicroX, as demonstrated at ID29, will quickly find its broad applicability at upcoming 4th generation synchrotron sources worldwide and opens a new frontier in time-resolved SmicroX. Advancing macromolecular structure determination with microsecond X-ray pulses at a 4th generation synchrotron.,Orlans J, Rose SL, Ferguson G, Oscarsson M, Homs Puron A, Beteva A, Debionne S, Theveneau P, Coquelle N, Kieffer J, Busca P, Sinoir J, Armijo V, Lopez Marrero M, Felisaz F, Papp G, Gonzalez H, Caserotto H, Dobias F, Gigmes J, Lebon G, Basu S, de Sanctis D Commun Chem. 2025 Jan 7;8(1):6. doi: 10.1038/s42004-024-01404-y. PMID:39775172 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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