Structural highlights
Function
FOCA_ECOLI Involved in the bidirectional transport of formate during mixed-acid fermentation (PubMed:23335413, PubMed:24659605, PubMed:30247527, PubMed:33169422, PubMed:35084298, PubMed:35377837, PubMed:35390794, PubMed:8022272). Functions to maintain relatively constant intracellular formate levels during growth, using different mechanisms for efflux and uptake of the anion (PubMed:35377837). Has a strong preference for formate as a substrate in vivo and not other acidic fermentation products (PubMed:23335413).[1] [2] [3] [4] [5] [6] [7] [8]
References
- ↑ Beyer L, Doberenz C, Falke D, Hunger D, Suppmann B, Sawers RG. Coordination of FocA and pyruvate formate-lyase synthesis in Escherichia coli demonstrates preferential translocation of formate over other mixed-acid fermentation products. J Bacteriol. 2013 Apr;195(7):1428-35. PMID:23335413 doi:10.1128/JB.02166-12
- ↑ Hunger D, Doberenz C, Sawers RG. Identification of key residues in the formate channel FocA that control import and export of formate. Biol Chem. 2014 Jul;395(7-8):813-25. PMID:24659605 doi:10.1515/hsz-2014-0154
- ↑ Hakobyan B, Pinske C, Sawers G, Trchounian A, Trchounian K. pH and a mixed carbon-substrate spectrum influence FocA formate-driven H2 production in Escherichia coli. FEMS Microbiol Lett. 2018 Nov 1;365(21). PMID:30247527 doi:10.1093/femsle/fny233
- ↑ Kammel M, Hunger D, Sawers RG. The soluble cytoplasmic N-terminal domain of the FocA channel gates bidirectional formate translocation. Mol Microbiol. 2021 Apr;115(4):758-773. PMID:33169422 doi:10.1111/mmi.14641
- ↑ Kammel M, Trebbin O, Pinske C, Sawers RG. A single amino acid exchange converts FocA into a unidirectional efflux channel for formate. Microbiology (Reading). 2022 Jan;168(1):001132. PMID:35084298 doi:10.1099/mic.0.001132
- ↑ Kammel M, Sawers RG. The FocA channel functions to maintain intracellular formate homeostasis during Escherichia coli fermentation. Microbiology (Reading). 2022 Apr;168(4). PMID:35377837 doi:10.1099/mic.0.001168
- ↑ Kammel M, Trebbin O, Sawers RG. Interplay between the Conserved Pore Residues Thr-91 and His-209 Controls Formate Translocation through the FocA Channel. Microb Physiol. 2022;32(3-4):95-107. PMID:35390794 doi:10.1159/000524454
- ↑ Suppmann B, Sawers G. Isolation and characterization of hypophosphite--resistant mutants of Escherichia coli: identification of the FocA protein, encoded by the pfl operon, as a putative formate transporter. Mol Microbiol. 1994 Mar;11(5):965-82. PMID:8022272 doi:10.1111/j.1365-2958.1994.tb00375.x
