9j03 | pdb_00009j03

Cyro-EM Structure of Human TLR4/MD-2/DLAM1 Complex

spinqualitylabelsall models 9j03, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9j03 is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.7Å Ligands: 2IL, BMA, GP4, NAG, X6Z Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function B3Y6A6_PANTR Publication Abstract from PubMed The mammalian pattern-recognition receptor TLR4/MD-2 (Toll-like receptor 4/myeloid differentiation factor-2) can be activated by a wide variety of pathogen-associated and endogenous molecules, with Gram-negative bacterial lipopolysaccharide (LPS) being the primary natural TLR4 agonist. Activation of TLR4 triggers cellular signaling that enables the beneficial innate immune responses and enhances adaptive immunity, thereby emphasizing the potential of TLR4 agonists for the management of diseases with an immunopathological background and for use as vaccine adjuvants. Given the challenges associated with LPS-derived products, including structural complexity, heterogeneity, toxicity, and species specificity, synthetic molecules targeting TLR4/MD-2 offer a promising alternative. Here, we elucidate the structural basis for the recognition of synthetic LPS-mimicking glycolipids, Disaccharide Lipid A Mimetics (DLAMs), by human and mouse TLR4/MD-2 through cryo-EM structures of six dimeric [TLR4/MD-2/ligand](2) complexes resolved at 2.2-3.1 A. We reveal that the specific binding modes of DLAMs, distinct from those of LPS, are essential for the species-independent TLR4 agonistic activity. DLAMs function as a molecular bridge, effectively induce the dimerization of TLR4/MD-2 complexes through specific carbohydrate structure-relevant ligand-protein interactions. Our findings reveal the distinct molecular modes of TLR4 activation, and provide a structural basis for the rationale design and development of innovative, highly potent TLR4-targeting immunotherapeutics and adjuvants. Structural insight into TLR4/MD-2 activation by synthetic LPS mimetics with distinct binding modes.,Fu Y, Kim H, Lee DS, Han AR, Heine H, Zamyatina A, Kim HM Nat Commun. 2025 May 5;16(1):4164. doi: 10.1038/s41467-025-59550-3. PMID:40325026 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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