9md6

Neuraminidase in complex with mAb 6-23.1

spinqualitylabelsall models 9md6, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9md6 is a 12 chain structure with sequence from Influenza A virus and Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 2.7Å Ligands: BMA, CA, MAN, NAG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Antibodies are crucial therapeutics, comprising a substantial portion of approved drugs due to their safety and clinical efficacy. Traditional antibody discovery methods are labor-intensive, limiting scalability and high-throughput analysis. Here, we improved upon our streamlined approach combining structural analysis and bioinformatics to infer heavy and light chain sequences from cryo-EM (cryo-electron microscopy) maps of serum-derived polyclonal antibodies (pAbs) bound to antigens. Using ModelAngelo, an automated structure-building tool, we accelerated pAb sequence determination and identified sequence matches in B cell repertoires via ModelAngelo-derived hidden Markov models (HMMs) associated with pAb structures. Benchmarking against results from a nonhuman primate HIV vaccine trial, our pipeline reduced analysis time from weeks to under a day with higher precision. Validation with murine immune sera from influenza vaccination revealed multiple protective antibodies. This workflow enhances antibody discovery, enabling faster, more accurate mapping of polyclonal responses with broad applications in vaccine development and therapeutic antibody discovery. Functional and epitope specific monoclonal antibody discovery directly from immune sera using cryo-EM.,Ferguson JA, Raghavan SSR, Alzua GP, Bhavsar D, Huang J, Rodriguez AJ, Torres JL, Bottermann M, Han J, Krammer F, Batista FD, Ward AB Sci Adv. 2025 Aug 15;11(33):eadv8257. doi: 10.1126/sciadv.adv8257. Epub 2025 Aug , 15. PMID:40815640 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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