9mne | pdb_00009mne

Crystal structure of enteropathogenic Escherichia coli EspC

spinqualitylabelsall models 9mne, resolution 2.94Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 9mne is a 2 chain structure with sequence from Escherichia coli O127:H6 str. E2348/69. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: X-ray diffraction, Resolution 2.94Å Ligands: GOL, P6G, PE8, PEG Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function ESPC_ECO27 Serine protease with enterotoxic and cytotoxic activities. Cleaves fodrin, but does not cause its redistribution within epithelial cells. The exact role of EspC in EPEC pathogenesis is still unknown. Publication Abstract from PubMed Enteropathogenic E. coli (EPEC) is a significant cause of diarrhea, leading to high infant mortality rates. A key toxin produced by EPEC is the EspC autotransporter, which is regulated alongside genes from the locus of enterocyte effacement (LEE), which collectively result in the characteristic attaching and effacing lesions on the intestinal epithelium. In this study, we present the crystal structure of the EspC passenger domain (alpha(EspC)) revealing a toxin comprised a serine protease attached to a large beta-helix with additional subdomains. Using various modified EspC expression constructs, alongside type III secretion system-mediated cell internalization assays, we dissect how the alpha(EspC) structural features enable toxin entry into the intestinal epithelium to cause cell cytotoxicity. The crystal structure of the toxin EspC from enteropathogenic Escherichia coli reveals the mechanism that governs host cell entry and cytotoxicity.,Pilapitiya AU, Hor L, Pan J, Wijeyewickrema LC, Pike RN, Leyton DL, Paxman JJ, Heras B Gut Microbes. 2025 Dec;17(1):2483777. doi: 10.1080/19490976.2025.2483777. Epub , 2025 Mar 31. PMID:40164999 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

Proteopedia Page Contributors and Editors (what is this?)

OCA