9t1d | pdb_00009t1d
Cryo-EM reconstruction of undecorated GDP microtubule
Structural highlights
FunctionTBA1A_PIG Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. Publication Abstract from PubMedMicrotubules are cytoskeletal filaments that are typically characterized by a discontinuous helical lattice of alpha/beta-tubulin heterodimers. Microtubules can also adopt variable lattice architectures both in vitro and in cellular contexts. Pseudo-helical averaging processing strategies have been developed to generate cryo-EM reconstructions of microtubules with and without decorating protein-binding partners, but these pipelines can be difficult to implement for the average user, especially for undecorated filaments. Here, we describe MiCSPARC, a cryo-EM processing pipeline developed around CryoSPARC [Punjani et al. (2017), Nat. Methods, 14, 290-296], which leverages automated particle picking and fast 3D refinement times in CryoSPARC to determine the structures of both decorated and undecorated microtubules. We generate reconstructions of undecorated GDP microtubules, as well as kinesin-1 motor domain-decorated GMPCPP filaments, at resolutions of up to 2.8 A, demonstrating the robustness of the pipeline. Based on its convenient implementation and its ability to routinely generate high-resolution, seam-corrected microtubule reconstructions, MiCSPARC should provide a valuable tool for understanding microtubule dynamics, microtubule-associated proteins and microtubule-targeting agents. A cryo-EM processing pipeline for microtubules using CryoSPARC.,Zhang D, Munoz-Hernandez H, Filipcik P, Sejwal K, Xu Y, Choi SR, Steinmetz MO, Wieczorek M Acta Crystallogr D Struct Biol. 2026 May 1;82(Pt 5):411-420. doi: , 10.1107/S2059798326003062. Epub 2026 Apr 27. PMID:42044018[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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