9wd2
Thymidylate kinase of Helicobacter pylori
Structural highlights
FunctionKTHY_HELPY Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis. Publication Abstract from PubMedThymidine (deoxythymidine triphosphate) plays very important role in DNA synthesis, replication and repair. Therefore, thymidine synthesis pathway enzymes are crucial for the survival of the organism and hence are potent therapeutic targets. Thymidylate kinase (TMPK) is at the junction of de novo synthesis pathway and salvage pathway of thymidine synthesis. TMPK is widely recognized as a potential therapeutic target. Inhibiting TMPKs would be an effective technique for discovering medications to treat infectious disorders like bacterial and parasite infections. The slight variation in active sites between human TMPK (hTMPK) and pathogen TMPKs provide support for the development of specific inhibitors. Here, we report the crystal structure of thymidylate kinase from Helicobacter pylori (HpTMPK) at 2.5 A. The three-dimensional structure of HpTMPK depicts two conserved regions DRX motif and P loop. The highly flexible LID region was absent in HpTMPK crystal structure. Chemdiv library was screened against HpTMPK and the compounds were shortlisted based on the docking scores. Our in-vitro enzyme inhibition study shows that compound F725_0025 exhibits the best inhibition with an IC(50) of 84 microM and a strong affinity of 10.7 microM. It inhibits Helicobacter pylori (H. pylori) with an IC(50) value of 30.14 microM. According to the growth curve of H. Pylori in the presence of inhibitory chemicals, F725_0025 may be a promising lead therapeutic molecule to combat H. pylori infection. Targeting Helicobacter pylori thymidylate kinase: structural insights and validation of novel inhibitors.,Kumari K, Khan FM, Bose M, Ls RJ, Yadav A, Mondal N, Gourinath S J Struct Biol. 2026 Jun;218(2):108319. doi: 10.1016/j.jsb.2026.108319. Epub 2026 , Apr 2. PMID:41935652[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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