2olp | pdb_00002olp
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Structure and ligand selection of hemoglobin II from Lucina pectinata
Overview
Haemoglobin II is one of three haemoglobins present in the cytoplasm of, the Lucina pectinata mollusc that inhabits the Caribbean coast. Using HBII, purified from its natural source, crystallization screening was performed, using the counter-diffusion method with capillaries of 0.2 mm inner, diameter. Crystals of HbII suitable for data collection and structure, determination were grown in the presence of agarose at 0.1%(w/v) in order, to improve their quality. The crystals belong to the tetragonal space, group P4(2)2(1)2, with unit-cell parameters a = b = 73.92, c = 152.35 A, and diffracted X-rays to a resolution of better than 2.0 A. The asymmetric, unit is a homodimer with a corresponding Matthews coefficient (VM) of 3.15, A3 Da(-1) and a solvent content of 61% by volume.
About this Structure
2OLP is a Single protein structure of sequence from Lucina pectinata with SO4, HEM and OXY as ligands. Full crystallographic information is available from OCA.
Reference
Capillary crystallization and molecular-replacement solution of haemoglobin II from the clam Lucina pectinata., Gavira JA, de Jesus W, Camara-Artigas A, Lopez-Garriga J, Garcia-Ruiz JM, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Mar 1;62(Pt, 3):196-9. Epub 2006 Feb 10. PMID:16511300
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