2uwi | pdb_00002uwi
STRUCTURE OF CRME, A POXVIRUS TNF RECEPTOR
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Overview
Vaccinia virus (VACV), the smallpox vaccine, encodes many proteins that, subvert the host immune response. One of these, cytokine response modifier, E (CrmE), is secreted by infected cells and protects these cells from, apoptotic challenge by tumour necrosis factor alpha (TNFalpha). We have, expressed recombinant CrmE from VACV strain Lister in Escherichia coli, shown that the purified protein is monomeric in solution and competent to, bind TNFalpha, and solved the structure to 2.0 A resolution. This is the, first structure of a virus-encoded tumour necrosis factor receptor (TNFR)., CrmE shares significant sequence similarity with mammalian type 2 TNF, receptors (TNFSFR1B, p75; TNFR type 2). The structure confirms that CrmE, adopts the canonical TNFR fold but only one of the two "ligand-binding", loops of TNFRSF1A is conserved in CrmE, suggesting a mechanism for the, higher affinity of poxvirus TNFRs for TNFalpha over lymphotoxin-alpha. The, roles of dimerisation and pre-ligand-assembly domains (PLADs) in poxvirus, and mammalian TNFR activity are discussed.
About this Structure
2UWI is a Single protein structure of sequence from Vaccinia virus. Full crystallographic information is available from OCA.
Reference
Structure of CrmE, a Virus-encoded Tumour Necrosis Factor Receptor., Graham SC, Bahar MW, Abrescia NG, Smith GL, Stuart DI, Grimes JM, J Mol Biol. 2007 Sep 21;372(3):660-71. Epub 2007 Jul 3. PMID:17681535
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