2uy1 | pdb_00002uy1
CRYSTAL STRUCTURE OF CSTF-77
|
Overview
The cleavage stimulation factor (CstF) is essential for the first step of, poly(A) tail formation at the 3' ends of mRNAs. This heterotrimeric, complex is built around the 77-kDa protein bridging both CstF-64 and, CstF-50 subunits. We have solved the crystal structure of the 77-kDa, protein from Encephalitozoon cuniculi at a resolution of 2 A. The, structure folds around 11 Half-a-TPR repeats defining two domains. The, crystal structure reveals a tight homodimer exposing phylogenetically, conserved areas for interaction with protein partners. Mapping experiments, identify the C-terminal region of Rna14p, the yeast counterpart of, CstF-77, as the docking domain for Rna15p, the yeast CstF-64 homologue.
About this Structure
2UY1 is a Single protein structure of sequence from Encephalitozoon cuniculi. Full crystallographic information is available from OCA.
Reference
The structure of the CstF-77 homodimer provides insights into CstF assembly., Legrand P, Pinaud N, Minvielle-Sebastia L, Fribourg S, Nucleic Acids Res. 2007;35(13):4515-22. Epub 2007 Jun 21. PMID:17584787
Page seeded by OCA on Wed Jan 23 14:41:53 2008
