2oga | pdb_00002oga
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X-ray crystal structure of S. venezuelae DesV in complex with ketimine intermediate
Overview
Desosamine is a 3-(dimethylamino)-3,4,6-trideoxyhexose found in certain, macrolide antibiotics such as the commonly prescribed erythromycin. Six, enzymes are required for its biosynthesis in Streptomyces venezuelae. The, focus of this article is DesV, which catalyzes the PLP-dependent, replacement of a 3-keto group with an amino functionality in the fifth, step of the pathway. For this study the three-dimensional structures of, both the internal aldimine and the ketimine intermediate with glutamate, were determined to 2.05 A resolution. DesV is a homodimer with each, subunit containing 12 alpha-helical regions and 12 beta-strands that, together form three layers of sheet. The structure of the internal, aldimine demonstrates that the PLP-cofactor is held in place by residues, contributed from both subunits (Asp 164 and Gln 167 from Subunit I and Tyr, 221 and Asn 235 from Subunit II). When the ketimine intermediate is, present in the active site, the loop defined by Gln 225 to Ser 228 from, Subunit II closes down upon the active site. The structure of DesV is, similar to another sugar-modifying enzyme referred to as PseC. This enzyme, is involved in the biosynthesis of pseudaminic acid, which is a sialic, acid-like nonulosonate found in the flagellin of Helicobacter pylori. In, the case of PseC, however, the amino group is transferred to the C-4, rather than the C-3 position. Details concerning the structural analysis, of DesV and a comparison of its molecular architecture to that of PseC are, presented.
About this Structure
2OGA is a Single protein structure of sequence from Streptomyces venezuelae with CL, NA, EDO and PGU as ligands. Full crystallographic information is available from OCA.
Reference
Molecular architecture of DesV from Streptomyces venezuelae: a PLP-dependent transaminase involved in the biosynthesis of the unusual sugar desosamine., Burgie ES, Thoden JB, Holden HM, Protein Sci. 2007 May;16(5):887-96. PMID:17456741
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