Replication Termination Protein
Replication Termination Proteins
Rtp and Tus These polar fork bocking sites have been found in yeast, pea, frog and human genomes.
RTP
RTP is a DNA binding protein from Bacillus Subtilis that uses a helix-loop-helix binding motif. In solution it shows a symmetric structure typical of the winged helix loop helix family, with an unstructured N-terminus end, first alpha helix (a1), unstructured loop that is equivalent to the first beta sheet (B1), helix loop helix structure (a2-a3), 2 beta sheets with a connecting loop that makes up the 'wing' structure (B2 - B3) and an additional long alpha helix involved in dimerisation (a4).
Assymmetry of RTP in DNA-binding
Wing-up: Contacts upstream with rtp dimer bound to A-site
Wing-down: Contacts with phosphate backbone of downstream DNA
Residues binding in both RTP monomers: Gln15, Arg16, Leu35, Asn53, His54, Thr55, Tyr58, Arg59, His62, gln72 (RED) Residues binding in only wing down position: Lys14, Tyr33, Lys77, Gln83, Val86 (BLUE) Residues binding in only wing up position: Thr9, Lys36, Glu56 (GREEN)
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Craig Mooney, Michal Harel, Joel L. Sussman, Alexander Berchansky
