348d | pdb_0000348d
X-RAY CRYSTAL STRUCTURES OF THE DECAMER DGACCGCGGTC: LOW SALT CONCENTRATION
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Overview
The X-ray crystal structure of the DNA decamer d(GACCGCGGTC), containing, half the human papilloma virus E2 binding site, has been solved from two, crystals grown at different ionic conditions (50 mM MgCl2and 50 mM, spermine or 1.56 mM MgCl2and 1.56 mM spermine). Despite the variation in, salt concentration, the two DNA structures are in a very similar, A-type, DNA conformation, with helical axes curving towards the major groove., Although the salt concentrations do not effect the helical parameters or, hydration to a large degree, there is a change in the overall helical, curvature; 18 degrees and 31 degrees for the low and high salt structures, respectively. This curvature appears to be sequence specific and, biologically relevant when compared with similar DNA structures, including, the E2 binding site of a protein-DNA complex.
About this Structure
348D is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
X-ray crystal structures of half the human papilloma virus E2 binding site: d(GACCGCGGTC)., Finley JB, Luo M, Nucleic Acids Res. 1998 Dec 15;26(24):5719-27. PMID:9838004
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