User:Mitchell Long/Sandbox 1

spinqualitylabelsall models Structure of Bacterial Luciferase and FMN complex from Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Introduction Luciferases are a class of enzymes that catalyze the oxidation of a long chain aliphatic aldehydes. This reaction results in the formation of a carboxylic acid and the emission of photons in the form of blue-green light.

spinqualitylabelsall models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D load='3fgc' size='350' frame='false' scene=
3fgc, resolution 2.30Å (default scene)
Ligands:	FMN, PO4, SO4
Gene:	luxA (Vibrio harveyi), luxB (Vibrio harveyi)
Activity:	Alkanal monooxygenase (FMN-linked), with EC number 1.14.14.3
Related:	1luc, 1brl
Structural annotation: Resources: InterPro : Ipr011251, Ipr016048, Ipr002103, Ipr018235 Pfam : PF00296 UniProt : P07740, P07739
Evolutionary conservation: Toggle Conservation Colors: Rows = identical sequences: Further Information: Caveats, Explanation, Complete Results at ConSurfDB
Resources:	FirstGlance, OCA, RCSB, PDBsum
Coordinates:	save as pdb, mmCIF, xml

Mechanism of Bio luminescence

Luciferase found in 'Italic tex

spinqualitylabelsall models Structure of Bacterial Luciferase and FMN complex from Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Introduction Luciferases are a class of enzymes that catalyze the oxidation of a long chain aliphatic aldehydes. This reaction results in the formation of a carboxylic acid and the emission of photons in the form of blue-green light.

spinqualitylabelsall models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D load='3fgc' size='350' frame='false' scene=
3fgc, resolution 2.30Å (default scene)
Ligands:	FMN, PO4, SO4
Gene:	luxA (Vibrio harveyi), luxB (Vibrio harveyi)
Activity:	Alkanal monooxygenase (FMN-linked), with EC number 1.14.14.3
Related:	1luc, 1brl
Structural annotation: Resources: InterPro : Ipr011251, Ipr016048, Ipr002103, Ipr018235 Pfam : PF00296 UniProt : P07740, P07739
Evolutionary conservation: Toggle Conservation Colors: Rows = identical sequences: Further Information: Caveats, Explanation, Complete Results at ConSurfDB
Resources:	FirstGlance, OCA, RCSB, PDBsum
Coordinates:	save as pdb, mmCIF, xml

Mechanism of Bio luminescence

Luciferase found in 'Vibrio harveyi' is a heterodimeric flavin monoxygenase that is composed of a catalytic α subunit and a homologous but noncatalytic β subunit. The catalytic α subunit is bound to a Flavin Mononucleotide cofactor. Bioluminescense is achieved by a two step reaction between

Structural Motifs

Active Site

Mobile Loop-The mobile loop of luciferase is thought to be responsible for protecting reaction intermediates and preventing the entry of bulk solvent into the active center once the flavin substrate is bound.

(β/α)&sub8; Barrel-

Interface between Alpha and Beta Subunits

Applications In Biotechnology

The luciferase assay

Quorum Sensing

Quorum sensing is a population density dependent form gene expression found in many bacteria.

Proteopedia Page Contributors and Editors (what is this?)

Mitchell Long